Cristiana Boi

TL;DR: This review considers the use of membrane adsorbers as an alternative technology for capture and polishing steps for the purification of monoclonal antibodies.

TL;DR: The proposed model provides a new insight into the phenomena involved in the adsorption on affinity membranes and it is a valuable tool to assess the use of membrane adsorbers in large scale processes.

TL;DR: A mathematical model is proposed for the description of protein purification through membrane affinity chromatography that takes into account convection, axial dispersion and binding reaction kinetics in the porous membrane matrix while boundary layer mass transfer resistance is shown to be negligible.

TL;DR: New affinity membranes endowed with an interesting binding capacity for human IgG are studied in view of their application in the capturing step of a monoclonal antibody production process, and appear good candidates to avoid the throughput limitations and other well‐known drawbacks of traditional bead‐based chromatographic columns.

TL;DR: The comparison was performed using anion exchange media with the same ligand immobilized on the adsorbing surface, namely the strong Q quaternary ammonium group, R-CH2-N+-(CH3)3, and bovine serum albumin as a model protein to confirm the better performance of membrane chromatography at high flow rates.