Showing papers in "Journal of Chromatography B in 2007"

TL;DR: This paper describes the key elements of a flexible, generic downstream process platform for mAbs that has been adopted at Amgen and consists of a well-defined sequence of unit operations with most operating parameters being pre-defined and a small subset of parameters requiring development effort.

TL;DR: The combination of polymeric mixed-mode SPE, the appropriate mobile phase pH and UPLC technology provides significant advantages for reducing matrix effects resulting from plasma matrix components and in improving the ruggedness and sensitivity of bioanalytical methods.

TL;DR: The present article gives an overview of the currently available assays of nitrite and nitrate in biological fluids based on the Griess reaction, the most frequently used analytical approach to quantitate the major metabolites of NO, i.e. nitriteand nitrate, in a variety of biological fluids, notably blood and urine.

TL;DR: A minimized SPA derivative has been constructed and a domain originating from SPA has been improved to withstand the harsh environment employed in industrial purifications.

TL;DR: This study presents a validated liquid chromatography technique coupled with tandem mass spectrometry (LC-MS/MS) to measureCurcumin in rat plasma and provide curcuminoids analysis from the extract of Curcumin longa L. longa to investigate the pharmacokinetics of curcuming in a freely moving rat.

TL;DR: A wide survey of technologies that are competing to be part of a platform, and an assessment of those that have the most promise are provided.

TL;DR: The potential and limitations of tandem mass spectrometry of glycopeptides as a tool in structural glycoproteomics are discussed.

TL;DR: This method simultaneously determines epinephrine, norepinephrine, dopamine and 5-hydroxytryptamine by HPLC coupled to atmospheric pressure chemical ionization mass spectrometry, using bovine chromaffin cells to test xenobiotic neurotoxicity and the secretion alterations of these neurotransmitters as endpoint.

TL;DR: Although it is possible to measure these 22 phthalate metabolites with adequate precision and accuracy at sub-parts-per-billion levels, additional information, including toxicokinetic data, is needed to demonstrate the usefulness of these phthalates for exposure assessment purposes.

TL;DR: This paper focuses on the process economic issues associated with manufacturing antibodies and reviews the cost studies published in the literature and identifies the key economic drivers, such as fermentation titre and overall yield, which are critical determinants of economic success.

TL;DR: In this review, problems associated with general strategies of samples preparation, and experimental demands for these processes are discussed.

TL;DR: The goal of this article is to review existing methodologies for the measurement of nitrite and RSNO by reductive chemiluminescence using tri-iodide and copper(I)/cysteine-based reduction methods coupled to chemilUMinescence.

TL;DR: This HPLC method is well suited for high-throughput quantitative determination of aminothiols in clinical studies, and is currently being used to support investigations of oxidative stress in patients with chronic kidney disease.

TL;DR: Investigation of Met oxidation of a fully human recombinant monoclonal antibody under both chemically stressed conditions using tert-butylhydroperoxide and thermal stability conditions revealed that the susceptible Met residues of each heavy chain were randomly oxidized in samples incubated with tBHP, while in the thermal stability sample, the susceptibleMet residues of oneheavy chain were preferentially oxidized.

TL;DR: A reversed-phase HPLC method that uses gradient elution and UV detection (325 nm) to determine levels of resveratrol and identify six major conjugated metabolites in the plasma and urine of human volunteers after administration of a single oral dose of 1g is described.

TL;DR: The results of matrix-assisted laser desorption/ionization (MALDI) imaging and direct molecular identification using tandem mass spectrometry (MS/MS) in colon cancer liver metastasis show the feasibility of MALDI imaging as a tool for the analysis of pathological specimens.

TL;DR: The more important benzimidazoles are introduced and their pharmacological effects and physiochemical properties discussed and the metabolism of these drugs is described relating to the occurrence and persistence of residues in biological matrices, providing information for selection of suitable matrices and target residues for testing.

TL;DR: A simple, rapid, precise and accurate isocratic reversed-phase stability-indicating HPLC method was developed and validated for the simultaneous determination of atorvastatin (AT) and amlodipine (AM) in commercial tablets and has shown adequate separation for AM, AT from their associated main impurities and their degradation products.

TL;DR: This review will describe the protocols applied to prepare 2D gels properly, and analyse and summarise the major challenges for successful proteome analysis using 2DE, i.e. the ability to analyse very alkaline, hydrophobic and/or low or high M(r) proteins with high resolution and the able to detect minor components.

TL;DR: The developed fingerprint assay could be readily utilized as a quality control method for S. miltiorrhiza and its related preparations and was the first time to identify 34 peaks by comparing with standard compounds and using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS(n) technique.

TL;DR: Findings suggest that although all the plants studied are from the same Compositae family, their flavonoids and phenolic compositions are markedly different, and the proposed method is useful for further chromatographic fingerprinting of plant Flavonoids.

TL;DR: A LC-tandem MS method for the simultaneous determination of arginine, ADMA, and SDMA in plasma is reported, designed for high sample throughput of only 20-mul aliquots of human or mouse plasma.

TL;DR: HPLC-DAD-ESI-MS(n) was used to analyze flavonoids in the roots of S. baicalensis and found two C-glycosides, apigenin-6-C-glucyl-8- C-arabinoside and chrysin- 6,8-di-C -glucoside, together with some O-glyCosides, are reported from S. Baicalensis for the first time.

TL;DR: The method of combining 1D- and GC x GC-TOF/MS is useful for the metabolic phenotyping of natural variants in rice for further studies in breeding programs.

TL;DR: Procedures for sample cleanup, derivatization, and chromatographic separation of arginine and its methylated analogs are the main topics of this review and important aspects of method validation, pre-analytical factors, and reference values are discussed.

TL;DR: The recently developed fluorometric HPLC method, which involves pre-column derivatization of nitrite with 2,3-diaminonaphthalene (DAN) and the enzymatic conversion of nitrate into nitrite, offers the advantages of easy sample preparation, simple derivatized, stable fluorescent derivatives, rapid analysis, high sensitivity and specificity, lack of interferences, and easy automation.

TL;DR: This review considers the use of membrane adsorbers as an alternative technology for capture and polishing steps for the purification of monoclonal antibodies.

TL;DR: The method is used for determination of cardiovascular drugs in post-mortem whole blood samples from forensic autopsy cases and robustness tested by systematically searching for satisfactory conditions using experimental designs including factorial and response surface designs.

TL;DR: The proposed HPLC method can be used for the analysis and standardization of 6-, 8-, and 10-gingerol in ginger-containing dietary supplements, spices, food products and beverages and as a method for determining the amounts of 6-shogaol as a marker for 6-gingingerol stability.

TL;DR: The current analytical methods applied to support the purification of monoclonal antibodies are reviewed.