D. Segerbäck

TL;DR: The degree of alkylation of haemoglobin was determined at different times after treatment of mice with one directly active alkylating agent, ethylene oxide, and one agent that requires metabolic activation, dimethylnitrosamine, to provide a basis for the use ofHaemoglobin as a monitor for integral doses of genotoxic environmental chemicals.

TL;DR: In persons occupationally exposed to ethylene oxide, i.e. under the conditions described by Dunkelberg and Hartmetz (1977), the degree of alkylation in histidine of hemoglobin was determined and quantitative determination of N-3-(2-hydroxyethyl)histidine by mass fragmentography and by ion-exchange amino-acid analysis gave consistent results.

TL;DR: Male CBA mice, exposed to air contaminated with [14C] labelled e thene, were able to metabolize this olefine to ethene oxide and the amount of epoxide formed was quantitatively determined from the degree of alkylation of cysteine and histidine in haemoglobin.

TL;DR: Experiments with mice show that the pre-carcinogen vinyl chloride is metabolically converted to a short-lived alkylating intermediate which introduces the 2-oxoethyl group onto nucleophilic sites in DNA and proteins.

TL;DR: The present study explores the possibilities of using specific amino acids in haemoglobin for tissue dosimetry of alkylating agents and demonstrates a stability of these alkylated products, which is fundamental to their use as integral dose monitors.