D
Da-Fu Cui
Researcher at Chinese Academy of Sciences
Publications - 3
Citations - 144
Da-Fu Cui is an academic researcher from Chinese Academy of Sciences. The author has contributed to research in topics: Pichia pastoris & Insulin. The author has an hindex of 3, co-authored 3 publications receiving 142 citations.
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Human insulin from a precursor overexpressed in the methylotrophic yeast Pichia pastoris and a simple procedure for purifying the expression product.
Yan Wang,Zhen-He Liang,You-Shang Zhang,Shi-Yin Yao,Ying-Gao Xu,Yue-Hua Tang,Shangquan Zhu,Da-Fu Cui,You-Min Feng +8 more
TL;DR: In this article, the methylotrophic yeast Pichia pastoris was used to express an insulin precursor, which reached 1.5 g/L in high-density fermentation using a simple culture medium composed mainly of salt and methanol.
COMMUNICATION TO THE EDITOR Human Insulin from a Precursor Overexpressed in the Methylotrophic Yeast Pichia pastoris and a Simple Procedure for Purifying the Expression Product
Yan Wang,Zhen-He Liang,You-Shang Zhang,Shi-Yin Yao,Ying-Gao Xu,Yue-Hua Tang,Shangquan Zhu,Da-Fu Cui,You-Min Feng +8 more
TL;DR: The methylotrophic yeast Pichia pastoris, which proved successful in producing many heterologous proteins, was used to express an insulin precursor and human insulin with full receptor binding capacity and biological activity was obtained.
Journal Article
Secretory Expression of Human Insulin in Methylotrophic Yeast Pichia pastoris.
TL;DR: The porcine insulin precursor (PIP) gene and its derivative form sp-PIP gene, which had a nona-peptide (called spacer peptide, sp) added at the 5' terminus of PIP gene were inserted into the plasmid pPIC9 of Pichia pastoris to obtain secretory plasmids pPic9/PIP and pP IC9/sp-Pip, respectively.