Dalia Shallom-Shezifi

TL;DR: Structures of the enzyme-substrate (xylobiose) complex provide insights into the role of the three catalytic residues of the inverting family 43 beta-xylosidase, and why Asp128 is an invariant among all five-bladed beta-propeller glycoside hydrolases.

TL;DR: A series of new derivatives of the clinically used aminoglycoside antibiotic paromomycin were designed, synthesized, and their ability to read-through premature stop codon mutations was examined in both in vitro translation system and ex vivo mammalian cultured cells.

TL;DR: This work has used a combination of biochemical and structural analysis to compare and contrast the molecular mechanisms of action and the structure–activity relationships of a new synthetic aminoglycoside, NB33, and a structurally similar natural aminglycoside apramycin, and demonstrates the general molecular principles that determine the decreased selectivity of apramYcin for the prokaryotic decoding site, and the increased selectivity for NB33.

TL;DR: The designed structures show antibacterial activity superior to that of neomycin B against pathogenic and resistant strains, while in parallel they demonstrate poor substrate activity with APH(3')-IIIa.

TL;DR: The chromosomal gene aph(3′)-IIb, encoding an aminoglycoside 3′-phosphotransferase in Pseudomonas aeruginosa, was cloned and overexpressed in Escherichia coli and was shown to phosphorylate its substrates at the C-3′-OH position.