D
Dan Anafi
Researcher at Amgen
Publications - 3
Citations - 915
Dan Anafi is an academic researcher from Amgen. The author has contributed to research in topics: Synuclein Family & Mutant. The author has an hindex of 3, co-authored 3 publications receiving 870 citations.
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Journal ArticleDOI
Both Familial Parkinson’s Disease Mutations Accelerate α-Synuclein Aggregation
Linda O. Narhi,Stephen J. Wood,Shirley Steavenson,Yijia Jiang,Dan Anafi,Stephen Kaufman,Francis Hall Martin,Karen C. Sitney,Paul Denis,Jean-Claude Louis,Jette Wypych,Anja Leona Biere,Martin Citron +12 more
TL;DR: It is shown that both wild type and mutant α-synuclein form insoluble fibrillar aggregates with antiparallel β-sheet structure upon incubation at physiological temperature in vitro, and that aggregate formation is accelerated by both PD-linked mutations.
Journal ArticleDOI
Parkinson's Disease-associated α-Synuclein Is More Fibrillogenic than β- and γ-Synuclein and Cannot Cross-seed Its Homologs
Anja Leona Biere,Stephen J. Wood,Jette Wypych,Shirley Steavenson,Yijia Jiang,Dan Anafi,Frederick W. Jacobsen,Mark A. Jarosinski,Gay-May Wu,Jean-Claude Louis,Francis Hall Martin,Linda O. Narhi,Martin Citron +12 more
TL;DR: β- and γ-synuclein are intrinsically less fibrillogenic than α- Synuclein and cannot form mixed fibrils with α- synuclein, which may explain why they do not appear in the pathological hallmarks of PD, although they are closely related to α-syn nuclein and are also abundant in brain.
Journal ArticleDOI
In vitro stoichiometry of complexes between the soluble RANK ligand and the monoclonal antibody denosumab.
Kelly K. Arthur,John P. Gabrielson,Nessa Hawkins,Dan Anafi,Jette Wypych,Athena Nagi,John K. Sullivan,Pavel V. Bondarenko +7 more
TL;DR: The studies revealed that an assembly including 3 denosumab antibody molecules bound to 2 RANKL trimers (3D2R) is the most stable complex in DPBS at 37 °C, which differs from the 1:1 binding stoichiometry reported for RankL and osteoprotegerin (OPG), a soluble homodimeric decoy receptor which binds RANKl with high affinity.