D
Daniel Kolakofsky
Researcher at University of Geneva
Publications - 146
Citations - 10043
Daniel Kolakofsky is an academic researcher from University of Geneva. The author has contributed to research in topics: Sendai virus & RNA. The author has an hindex of 60, co-authored 146 publications receiving 9760 citations. Previous affiliations of Daniel Kolakofsky include Brigham and Women's Hospital & University of Utah.
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Journal ArticleDOI
Paramyxovirus RNA Synthesis and the Requirement for Hexamer Genome Length: the Rule of Six Revisited
Daniel Kolakofsky,Thierry Pelet,Dominique Garcin,Stéphane Hausmann,Joseph Curran,Laurent Roux +5 more
TL;DR: The template for paramyxovirus RNA synthesis is not naked RNA but the helical nucleocapsid core of the virus, in which each nucleoc Capsid protein is predicted to be associated with precisely 6 nucleotides.
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A highly recombinogenic system for the recovery of infectious Sendai paramyxovirus from cDNA: generation of a novel copy-back nondefective interfering virus.
TL;DR: In this paper, the authors recovered infectious Sendai virus (SeV) from full-length cDNA (FL-3) by transfecting this cDNA and pGEM plasmids expressing the nucleocapsid protein (NP), phosphoprotein and large proteins into cells infected with a vaccinia virus which expresses T7 RNA polymerase.
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An N-terminal domain of the Sendai paramyxovirus P protein acts as a chaperone for the NP protein during the nascent chain assembly step of genome replication.
TL;DR: This domain is required to form a stable complex with unassembled NP (P-NP0) and to prevent NP from assembling illegitimately, i.e., independently of the concurrent assembly of a nascent viral genome.
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Isolation and characterization of Sendai virus DI-RNAs.
TL;DR: When passaged at high multiplicity, four strains of Sendai virus all showed evidence that they contained defective interfering (DI) particles, indicating that the ends of the DI-RNA are complementary, and the implications of this finding in terms of the mechanism of genome replication are discussed.
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The 5' ends of Hantaan virus (Bunyaviridae) RNAs suggest a prime-and-realign mechanism for the initiation of RNA synthesis.
TL;DR: A prime-and-realign mechanism of chain initiation is suggested in which mRNAs are initiated with a G-terminated host cell primer and genomes with GTP, and after extension by one or a few nucleotides, the nascent chain realigns backwards by virtue of the terminal sequence repeats, before processive elongation takes place.