Showing papers in "Cell in 1976"

TL;DR: Over 80 hybrid Col El-DNA (E. coli), plasmid-bearing clones have been identified in the colony bank, and about 40 known E. coli genes have been tentatively assigned to these various plasmids.

TL;DR: Starting with a clone converting to adipose cells with a vary low frequency, it has been possible, by serial selection, to generate subclones which convert with a high frequency, due to spontaneous heritable changes affecting susceptibility to the adipose conversion.

TL;DR: The physiologic importance of the cell surface LDL receptor was emphasized by the finding that mutant fibroblasts from a subject with homozygous Familial Hypercholesterolemia did not show cell surface binding of 125I-LDL, as measured by heparin release, at either 4 degrees C or 37 degrees C.

TL;DR: The results are consistent with the concept, deveolped from work on other cell types, that plasminogen activator may represent a generalized mechanism for tissue remodeling and cell migration.

TL;DR: Multiple forms of actin have been found in a variety of mammalian cell lines and tissues by the use of high resolution, two-dimensional gel electrophoresis, andryptic peptide comparisons have shown that muscle actin is distinguished from the two "nonmuscle" actins by several peptide differences, and that the two non-muscle actins are nearly identical.

TL;DR: Strain XB, an established keratinocyte line of mouse teratomal origin, also forms cornified envelopes, but the kinetics of the process are different, indicating that the program of terminal differentiation is not initiated at corresponding times in the two cell types.

TL;DR: The data strongly suggest that the rotation of the Watson Crick helix which accompanies ethidium intercalation causes the loss of nucleosomes from the DNA.

TL;DR: High molecular weight DNA, isolated from eleven cloned lines of rat cells independently transformed by SV40, was cleaved with various restriction endonucleases and the location of viral sequences among the fragments of transformed cell DNA was determined by autoradiography.

TL;DR: It was possible to show that the synthetic globin DNA is a faithful copy of β-globin mRNA by comparing the nucleotide sequences known or predicted from the amino acid sequences of α- and β-chain rabbit hemoglobin.

TL;DR: The potential roles of plasminogen activator in monocyte migration and the relationship of this enzyme to the anti-inflammatory effect of gluccorticoids are correlated and emphasized.

TL;DR: All rRNA loci, whether having complete or incomplete gradients, exhibit high densities of nascent transcripts per unit length, suggesting that the rate of chromatin transcription, rather than the RNA polymarase I pool size, limits rRNA synthesis on individual genes.

TL;DR: Tissue-specific differences in mRNA populations may be related to abundance as well as qualitative differences, and cross-hybridization experminets show that the most abundant class of mRNA in each tissue is characteristic, and that a high proportion of the total sequences are common between tissues.

TL;DR: The small molecular weight RNAs of the HeLa cell have been located in specific subcellular fractions and two short-lived cytoplasmic species behave kinetically as precursors to the stable nuclear RNAs.

TL;DR: A method for the clonal analysis of murine erythroleukemia cells has been developed which allows the precise characterization of the number of progeny produced by each cell and the degree of differentiation of each progeny cell, and it is observed that the commitment decision limits the subsequent proliferative capacity of the cell to four additional cell divisions.

TL;DR: It is concluded that although the other histones have a role in DNA organization within the nucleosome, the arginine-rich histone pair, H3/H4, can organize DNA segments the length of theucleosome core in the absence of all otherhistones.

TL;DR: Reassociation, hybridization, and restriction endonuclease studies, as well as electrophoretic analyses, indicate that the sequential actions of reverse transcriptase, DNA polymerase 1, and S1 nuclease generate full-length, double-stranded synthetic globin genes.

TL;DR: Two techniques were especially useful in the mapping: "cloning" 14S + 25S DNA on the plasmid pMB9 to amplify individual R1 fragments, and digesting DNA with R1 in the presence of the antibiotic distamycin A to produce specific partial digestion products.

TL;DR: Large differences in the extent of both mDNA and null mDNA reaction with the various RNAs were observed, indicating that in each state of differention a distinct set of structural genes is active, generally characterized by several thousand specific sequences.

TL;DR: Deoxyribonuclease I causes depolymerization of filamentous muscle actin to form a stable complex of 1 mole DNAase I:1 mole actin, and heavy meromyosin binds tightly to actin filaments and blocks completely the DNA enzyme I: actin filament interaction.

TL;DR: Saturation values are due to hybridization with nonrepeated DNA and are a direct measure of the sequence complexity of poly(A)mRNA, indicating that cytoplasmic polyadenylation does not occur to any apparent qualitative extent and that poly( a)m RNA is a subset of the poly (A)hnRNA population.

TL;DR: Virus-specific RNA was prepared from cells early after adenovirus type 2 infection and fractionated by hybridization to specific fragments of viral DNA to program cell-free protein synthesis, and the products were analyzed by electrophoresis.

TL;DR: These results show that pluripotency in embryonal carcinoma cells need not to be abolished by the introduction of a complete diploid genome from a differentiated cell.

TL;DR: When passaged at high multiplicity, four strains of Sendai virus all showed evidence that they contained defective interfering (DI) particles, indicating that the ends of the DI-RNA are complementary, and the implications of this finding in terms of the mechanism of genome replication are discussed.

TL;DR: The role of heme in erythroid development is investigated in ERYthroleukemic (Friend) cells, and Hemin and DMSO together cause T3-C12 cells to accumulate 8-9 fold more globin mRNA than either inducer individually.

TL;DR: In each of the three resistant clones tested, there is an increase in the number of aspartate transcarbamylase active sites, determined by titration with 3H-PALA, which closely parallels the increase in enzyme activity.

TL;DR: It is proposed that the basic unit of chromatin is constructed of two isologously paired heterotypic protein tetramers each containing one molecule of H2A, H2B, H3, and H4 histone, creating a nucleosome particle (DNA and protein) organized about a dyad axis of symmetry.

TL;DR: A convenient electron microscope label for mapping poly(A) sequences is synthesized and the RNAs of several type C oncornaviruses were examined by this method.