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Showing papers by "Daniel M. Brown published in 1996"

Journal Article•DOI•
An Approach to Random Mutagenesis of DNA Using Mixtures of Triphosphate Derivatives of Nucleoside Analogues

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Manuela Zaccolo, David M. Williams1, Daniel M. Brown1, Ermanno Gherardi•
Laboratory of Molecular Biology1
02 Feb 1996-Journal of Molecular Biology
TL;DR: A new method for random mutagenesis of DNA based on the use of a mixture of triphosphates of nucleoside analogues that enables very high frequencies of base substitutions and allows control of the mutational load via the number of DNA amplification cycles.

390 citations

Journal Article•DOI•
Enzymatic recognition of acyclic universal base analogues in oligonucleotides

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David Loakes, A Van Aerschotl, Daniel M. Brown, Fergal Hill
01 Jan 1996-Nucleosides, Nucleotides & Nucleic Acids
TL;DR: It is shown that acyclic sugars can function in place of the normal 2′- deoxyribose moiety with some enzymes, expanding the range of possible manipulations.
Abstract: The effects of the acyclic analogues 3 and 4 on recognition by commonly used enzymes in recombinant DNA work have been investigated. This report shows that acyclic sugars can function in place of the normal 2′- deoxyribose moiety with some enzymes, expanding the range of possible manipulations.

13 citations