Journal ArticleDOI
An Approach to Random Mutagenesis of DNA Using Mixtures of Triphosphate Derivatives of Nucleoside Analogues
TLDR
A new method for random mutagenesis of DNA based on the use of a mixture of triphosphates of nucleoside analogues that enables very high frequencies of base substitutions and allows control of the mutational load via the number of DNA amplification cycles.About:
This article is published in Journal of Molecular Biology.The article was published on 1996-02-02. It has received 390 citations till now. The article focuses on the topics: DNA & Mutagenesis.read more
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Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics
TL;DR: Aptamers are different from antibodies, yet they mimic properties of antibodies in a variety of diagnostic formats, and may play a key role either in conjunction with, or in place of, antibodies in the form of aptamer-based diagnostic products in the market.
Journal ArticleDOI
Tuning genetic control through promoter engineering
TL;DR: The characterized library of promoters is used to assess the impact of phosphoenolpyruvate carboxylase levels on growth yield and deoxy-xylulose-P synthase Levels on lycopene production and is illustrated as being generalizable to eukaryotic organisms and thus constitutes an integral platform for functional genomics, synthetic biology, and metabolic engineering endeavors.
Journal ArticleDOI
Isolating and engineering human antibodies using yeast surface display
Ginger Chao,Wai L. Lau,Benjamin J. Hackel,Stephen L. Sazinsky,Shaun M. Lippow,K. Dane Wittrup +5 more
TL;DR: This protocol describes the process of isolating and engineering antibodies or proteins for increased affinity and stability using yeast surface display using magnetic-activated cell sorting selection and flow cytometry to attain desired scFv properties through directed evolution.
Journal ArticleDOI
A Rapid, Reversible, and Tunable Method to Regulate Protein Function in Living Cells Using Synthetic Small Molecules
Laura A. Banaszynski,Ling chun Chen,Lystranne A. Maynard-Smith,A. G.Lisa Ooi,Thomas J. Wandless +4 more
TL;DR: A general technique to regulate the stability of specific proteins in mammalian cells using cell-permeable, synthetic molecules and genetic fusion of the destabilizing domain to a gene of interest ensures specificity, and the attendant small-molecule control confers speed, reversibility, and dose-dependence to this method.
Journal ArticleDOI
The 'evolvability' of promiscuous protein functions.
Amir Aharoni,Leonid Gaidukov,Olga Khersonsky,Stephen McQ Gould,Cintia Roodveldt,Dan S. Tawfik +5 more
TL;DR: Results from directed laboratory evolution experiments indicate that the evolution of a new function is driven by mutations that have little effect on the native function but large effects on the promiscuous functions that serve as starting point.
Related Papers (5)
Yeast surface display for screening combinatorial polypeptide libraries
Eric T. Boder,K D Wittrup +1 more