Daniel Stark

TL;DR: This review sums up the activity in the field of in situ product removal in whole cell bioprocesses over the last 20 years and emphasizes that the planning of a successful whole cell ISPR process should not only consider the choice of ISPR technique according to the physicochemical properties of the product, but also the potential configuration of the whole process set-up.

TL;DR: The production rates were limited by the intrinsic oxidative capacity of S. cerevisiae, and the high viscosity of the two‐phase system lowered the kla, and therefore also the productivity, so if a specific ISPR technique is planned, it consequently has to be remembered that the productivity of this bioconversion process is also quickly limited byThe kla of the fermenter at high cell densities.

TL;DR: Higher specific oxygen uptake ratesqO2 were found in the presence of the bioconversion at oxidative growth than the maximal respiratory capacity qO2max found in continuous cultures without bioconversions.

TL;DR: A novel in situ product removal (ISPR) method that uses microcapsules to extract inhibitory products from the reaction suspension is introduced into fermentation technology and the microbial cells are shielded from the phase toxicity of the organic solvent by a hydrogel layer surrounding the organic core.

TL;DR: Back to Basics: Thermodynamics in Biochemical Engineering, Integration of Physiology and Fluid Dynamics.