Showing papers by "David C. Schwartz published in 1984"
TL;DR: This pulsed field gradient gel electrophoresis fractionates intact S. cerevisiae chromosomal DNA, producing a molecular karyotype that greatly facilitates the assignment of genes to yeast chromosomes.
2,654 citations
TL;DR: The mini-chromosomes suggest that their main function is to provide a large pool of telomeric VSG genes, and the mini-exons encoding the 5' 35 nucleotides of VSG messenger RNAs are in large and 2 Mb DNA.
331 citations
Patent•
25 Sep 1984TL;DR: Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidification liquid and macromolecules such as DNA or intact chromosomes derived from the lyss, were used in this article.
Abstract: Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as DNA or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separations. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.
36 citations