David C. Thomas

TL;DR: The data suggest a PCNA requirement in mismatch repair at a step preceding DNA resynthesis in yeast and human expression libraries, and the ability of PCNA to bind to MLH1 and MSH2 may reflect linkage between mismatch repair and replication.

TL;DR: Parallel measurements of repair in HeLa extracts and in Escherichia coli suggest that repair specificities are similar for the two systems, and suggests that DNA polymerase alpha may function in mismatch repair.

TL;DR: In this paper, the authors compared cell-free extracts from RER+ endometrial and colorectal cancer cell lines to RER- cell lines, and found that the defect in these lines likely involves pre-incision events or the excision step, but not the incision, polymerization, or ligation steps.

TL;DR: The fidelity of SV40 origin-dependent DNA replication in human cell extracts is compared to the fidelity of mammalian DNA polymerases alpha, delta, and epsilon using lacZ alpha of M13mp2 as a reporter gene to demonstrate that exonucleolytic proofreading contributes to accuracy during synthesis by DNA polymerase delta.

TL;DR: Sequence analysis of mutants resulting from replication of UV-irradiated DNA demonstrated that most mutants contained C-->T transition errors at dipyrimidine sites, consistent with the interpretation that pyrimidine dimers are bypassed during replication by the multiprotein replication apparatus in human cell extracts.