Showing papers in "Journal of Biological Chemistry in 1991"

TL;DR: Peroxynitrite anion was a less effective thiol-oxidizing agent than its anion, with oxidation presumably mediated by the decomposition products, hydroxyl radical and nitrogen dioxide.

TL;DR: GF 109203X was a competitive inhibitor with respect to ATP and displayed high selectivity for PKC as compared to five different protein kinases, illustrating the potential of this compound as a tool for studying the involvement of PKC in signal transduction pathways.

TL;DR: This article showed that VEGF is produced by cultured vascular smooth muscle cells by polymerase chain reaction and cDNA cloning, and showed that the three forms of the human vascular endothelial growth factor (VEGF) protein chain predicted from these coding regions are 189, 165, and 121 amino acids in length.

TL;DR: In higher eukaryotes, translation is modulated at the level of initiation by five aspects of mRNA structure: (i) the m7G cap; (ii) the primary sequence or context surrounding the AUG codon; (iii) the position of the Aug codon, i.e. whether or not it s “first”; (iv) secondary structure both upstream and downstream from the AUU; and (v) leader length.

TL;DR: The expression of the TIS10 gene appears to be highly cell type-restricted in cultured cell lines; of 12 cell lines tested under superinducing conditions, only the rodent embryonic Swiss 3T3 and Rat1 cell lines expressed TIS12 gene.

TL;DR: The data suggest that the sequence, 5'-puGTGACNNNGC-3' 3'-pyCACTGNNNCG-5' where N is any nucleotide, represents the core sequence of the ARE required for transcriptional activation by phenolic antioxidants and metabolizable planar aromatic compounds.

TL;DR: The role of ATP-dependent calcium uptake into intracellular storage compartments is an essential feature of hormonally induced calcium signaling Thapsigargin, a non-phorboid tumor promoter, increasingly is being used to manipulate calcium stores because it induces a hormone-like elevation of cytosolic calcium as discussed by the authors.

TL;DR: Results suggest that the vacuolar type H(+)-ATPase plays a pivotal role in acidification and protein degradation in the lysosomes in vivo.

TL;DR: Findings suggest that disruption of the de novo pathway of sphingolipid biosynthesis may be a critical event in the diseases that have been associated with consumption of fumonisins.

TL;DR: It is indicated that a unique metabolic pathway exists in adipocytes that mediates desensitization of the insulin-responsive GTS, and that an early step in this pathway involves the conversion of fructose 6-phosphate to glucosamine 6- phosphate by the first and rate-limiting enzyme of the hexosamine pathway, glutamine:fructose-6-ph phosphate amidotransferase.

TL;DR: Investigation of the lipid fraction of rat epididymal adipocytes suggests that perilipin plays a role in the specialized lipid storage function of adipocytes, and Immunocytochemical studies with specific antiserum shows that the perilipIn is closely associated with the periphery of lipid storage droplets in cultured adipocytes.

TL;DR: It is proposed that the SpoT protein can either catalyze or control the alternative pathway of ppGpp synthesis in addition to its known role as a (p)ppGpp 3'-pyrophosphohydrolase.

TL;DR: It was possible to detect small amounts of esterified 24:5(n-3) and 24:6(n -3) in phospholipids, which is a finding consistent with their role as obligatory intermediates in 22:6 (n- 3) biosynthesis.

TL;DR: Results indicate that the conversion of PrP to the protease- and phospholipase-resistant state is a post-translational event that occurs after the precursor reaches the cell surface.

TL;DR: A concerted interplay among the domains of the AR protein in regulating gene transcription is demonstrated, with androgen-dependent nuclear uptake and inhibition of wild type AR by coexpression with an inactive NH2-terminal fragment suggested competition for nuclear factors required for transcriptional regulation.

TL;DR: Results support a mechanism in which N omega-hydroxy-L-arginine is generated as an intermediate in nitric oxide (.NO) synthesis through an NADPH-dependent hydroxylation of L-argInine.

TL;DR: Three proteins from barley (Hordeum vulgare L.) seeds which synergistically inhibit the growth of fungi measured in a microtiter well assay are purified and their biological roles and potential use in genetic engineering studies are discussed.

TL;DR: Results suggest that sphingomyelin turnover may be an important signaling mechanism transducing the actions of TNF alpha and gamma-IFN with specific function in cell differentiation.

TL;DR: Analysis of the derived amino acid sequence suggests that the protein contains four domains: a signal sequence, a catalytic domain, a serine/threonine-rich region, and a carboxyl-terminal domain with high binding affinity for chitin.

TL;DR: The P-450complex has a characteristic absorption maximum (Soret band) near 450 nm due to axial ligation with a cysteine thiolate of the protein (with or without substrate present).

TL;DR: Results indicate that the basic pair and the RXK/RR sequence are the signals for precursor cleavages catalyzed by PC3 within the regulated secretory pathway and by furin within the constitutive pathway, respectively.

TL;DR: In analyzing the glucocorticoid effects on PGHS synthesis in C127 mouse fibroblasts, a novel 4-kilobase mRNA is detected that is related to a PGHS cDNA cloned from an ovine seminal vesicle library and encodes a 70-kDa protein that is specifically immunoprecipitated by anti-PGHS serum.

TL;DR: It is demonstrated that a recombinant 39-k Da fusion protein can reversibly bind to the 515-kDa subunit of the LRP/alpha 2-macroglobulin receptor, which inhibits the binding and uptake of the receptor's two known ligands.

TL;DR: Adherence of HL-60 cells to human umbilical vein cell monolayers was inhibited by ISIS 1570 and ISIS 1939, demonstrating that the reduced levels of ICAM-1 impact on ICAM the emigration of leukocytes out of the vasculature.

TL;DR: Certain short peptide sequences in adhesion proteins are thought to serve as sites for recognition and binding by specific plasma membrane receptors in cells dependent on specific adhesive recognition sequences.