D
David Cheo
Researcher at Life Technologies
Publications - 12
Citations - 1142
David Cheo is an academic researcher from Life Technologies. The author has contributed to research in topics: Nucleic acid & Nucleic acid methods. The author has an hindex of 8, co-authored 12 publications receiving 1084 citations. Previous affiliations of David Cheo include Invitrogen.
Papers
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Journal ArticleDOI
Evaluation of quantitative miRNA expression platforms in the microRNA quality control (miRQC) study
Pieter Mestdagh,Nicole Hartmann,Lukas Baeriswyl,Ditte Andreasen,Nathalie Bernard,Caifu Chen,David Cheo,Petula D'Andrade,Mike DeMayo,Lucas Dennis,Stefaan Derveaux,Yun Feng,Stephanie Fulmer-Smentek,Bernhard Gerstmayer,Julia S. Gouffon,Chris Grimley,Eric Lader,Kathy Y. Lee,Shujun Luo,Peter Mouritzen,Aishwarya Narayanan,Sunali Patel,Sabine Peiffer,Silvia Rüberg,Gary P. Schroth,Dave Schuster,Jonathan M Shaffer,Elliot J Shelton,Scott Silveria,Umberto Ulmanella,Vamsi Veeramachaneni,Frank Staedtler,Thomas Peters,Toumy Guettouche,Linda Wong,Jo Vandesompele +35 more
TL;DR: The results indicate that each method for analysis of microRNA expression has its strengths and weaknesses, which help to guide informed selection of a quantitative microRNA gene expression platform for particular study goals.
Journal ArticleDOI
Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Jean François Rual,Tomoko Hirozane-Kishikawa,Tong Hao,Nicolas Bertin,Siming Li,Amélie Dricot,Ning Li,Jennifer Rosenberg,Philippe Lamesch,Pierre-Olivier Vidalain,Tracey R. Clingingsmith,James L. Hartley,Dominic Esposito,David Cheo,Troy Moore,Blake A. Simmons,Reynaldo Sequerra,Stephanie Bosak,Lynn Doucette-Stamm,Christian Le Peuch,Jean Vandenhaute,Michael E. Cusick,Joanna S. Albala,Joanna S. Albala,David E. Hill,Marc Vidal +25 more
TL;DR: The generation of a first version of the human ORFeome is described using a newly improved Gateway recombinational cloning approach, and the use of hORFeome v1.1 for heterologous protein expression in two different expression systems at proteome scale is described.
Journal ArticleDOI
Concerted Assembly and Cloning of Multiple DNA Segments Using In Vitro Site-Specific Recombination: Functional Analysis of Multi-Segment Expression Clones
TL;DR: In this article, the authors described the development of a novel technology that utilizes in vitro site-specific recombination to provide a robust and flexible platform for high-throughput cloning and transfer of DNA segments.
Patent
Use of multiple recombination sites with unique specificity in recombinational cloning
TL;DR: In this article, the authors present compositions and methods for recombinational cloning of two or more different nucleic acid molecules, which may be fused together or inserted into distinct sites in a vector.
Journal ArticleDOI
Feasibility of Genome-Scale Construction of Promoter::Reporter Gene Fusions for Expression in Caenorhabditis elegans Using a MultiSite Gateway Recombination System
Ian A. Hope,Jonathan Stevens,Anna Garner,Josie Hayes,David Cheo,Michael A. Brasch,Marc Vidal +6 more
TL;DR: A novel approach for linking multiple DNA segments, here applied to the generation of promoter::reporter fusions, is described, which allowed promoters from Caenorhabditis elegans genes to provide appropriate expression of GFP upon introduction into C. elegans.