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David J. Leak
Researcher at University of Bath
Publications - 127
Citations - 9692
David J. Leak is an academic researcher from University of Bath. The author has contributed to research in topics: Fermentation & Chemistry. The author has an hindex of 31, co-authored 118 publications receiving 8681 citations. Previous affiliations of David J. Leak include University of Warwick & National Technical University.
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The ribR gene encodes a monofunctional riboflavin kinase which is involved in regulation of the Bacillus subtilis riboflavin operon.
TL;DR: Measurement of flavokin enzyme activity in cell extracts demonstrated that ribR encodes a monofunctional flavokinase which converts rib oflavin into FMN but not to FAD, and is specific for the reduced form of riboflavin.
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Modular system for assessment of glycosyl hydrolase secretion in Geobacillus thermoglucosidasius.
TL;DR: A modular cassette for the inducible expression and secretion of proteins in G. thermoglucosidasius, designed to allow the simple interchange of parts, was demonstrated using the endoglucanase Cel5A from Thermotoga maritima as a secretion target.
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The microbial production of epoxides
TL;DR: The use of biocatalysts for production of epoxides as chiral intermediates in the synthesis of these compounds has realistic commercial prospects, but also presents formidable technical problems.
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Genetic tool development underpins recent advances in thermophilic whole‐cell biocatalysts
TL;DR: It is argued that the development of robust genetic systems is paramount in the evolution of future thermophilic based bioprocesses and suggestions for future approaches and genetic targets that will facilitate this process are made.
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Effect of nutrient limitation on product formation during continuous fermentation of xylose with Thermoanaerobacter ethanolicus JW200 Fe(7).
TL;DR: Thermoanaerobacter ethanolicus JW200 Fe(7) was grown in continuous culture, using xylose as the primary carbon source, with progressively lower concentrations of supplementary yeast extract, which enabled the comparison of metabolic flux to fermentation end-products under carbon-limited and carbon-sufficient conditions and the determination of process data under fully mass-balanced conditions.