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David J. Leak
Researcher at University of Bath
Publications - 127
Citations - 9692
David J. Leak is an academic researcher from University of Bath. The author has contributed to research in topics: Fermentation & Chemistry. The author has an hindex of 31, co-authored 118 publications receiving 8681 citations. Previous affiliations of David J. Leak include University of Warwick & National Technical University.
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Journal ArticleDOI
The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering.
TL;DR: A set of modular shuttle vectors, including a characterized promoter library and test ribosome binding site design, can facilitate modularity and part exchange to make Geobacillus a thermophile chassis for synthetic biology.
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An integrated biorefinery concept for conversion of sugar beet pulp into value-added chemicals and pharmaceutical intermediates
Max Cárdenas-Fernández,Maria Bawn,Charlotte Hamley-Bennett,Penumathsa K V Bharat,Fabiana Subrizi,Nurashikin Suhaili,David Ward,Sarah Bourdin,Paul A. Dalby,Helen C. Hailes,Peter Hewitson,Svetlana Ignatova,Cleo Kontoravdi,David J. Leak,Nilay Shah,Tom D. Sheppard,John M. Ward,Gary J. Lye +17 more
TL;DR: The technical feasibility of an integrated biorefinery concept for the fractionation of SBP and conversion of these monosaccharides into value-added products is described and the conversion of Ara into l-gluco-heptulose (GluHep), that has potential therapeutic applications in hypoglycaemia and cancer, using a mutant TK is described.
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Production of oligosaccharides and biofuels from Miscanthus using combinatorial steam explosion and ionic liquid pretreatment.
Rakesh Bhatia,Jai B. Lad,Maurice Bosch,David Bryant,David J. Leak,Jason P. Hallett,Telma Teixeira Franco,Joe Gallagher +7 more
TL;DR: In this article, the authors evaluated individual and combinatorial steam explosion (SE) and ionic liquid (IL) pretreatments for production of high-value oligosaccharides from a novel seed-based Miscanthus hybrid (Mx2779).
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In vivo studies of primary alcohols, aldehydes and carboxylic acids as electron donors for the methane mono-oxygenase in a variety of methanotrophs
David J. Leak,Howard Dalton +1 more
TL;DR: In type II methanotrophs, 5 mM-acetate, propionate and butyrate also stimulate methane mono-oxygenase activity apparently by inducing the breakdown of poly-β-hydroxybutyrate, which gives rise to NADH.
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Heterologous expression of pyruvate decarboxylase in Geobacillus thermoglucosidasius
TL;DR: It is demonstrated that Pdc from Zymomonas mobilis can be expressed in an active form in Geobacillusthermoglucosidasius at up to 52°C, while expression of Pdc polypeptides up to 54°C was evident from Western blotting.