David J. Pintel

TL;DR: The proposals introduce new species and genera into both subfamilies, resolve one misclassified species, and improve taxonomic clarity by employing a series of systematic changes.

TL;DR: Linear PEI is a better transfection reagent than calcium phosphate for the production of recombinant AAV in both HEK293 and HeLa cells, and when both HeLa and XDC293 cells were by the method, He La cells in the absence of E1A generated three-fold more recombination AAV than X DC293 cells, which constitutively express E1 A.

TL;DR: Members of the family Parvoviridae are small, resilient, non-enveloped viruses with linear, single-stranded DNA genomes of 4–6 kb, which are genetically limited and are host and/or tissue specific.

TL;DR: Results indicate that MVM DNA encodes two overlapping transcription units with separate promoters near the left end (4.0 mu) and middle of the genome and sequence analysis of these regions of the viral DNA reveal the characteristic features of RNA polymerase II promoters.

TL;DR: Results indicate that NS2 participates in MVM DNA replication and is required for efficient viral growth, and the requirement for NS2 during MVM replication is also host cell specific.