David P. Toczyski

TL;DR: An epistatic miniarray profile consisting of quantitative pairwise measurements of the genetic interactions between 743 Saccharomyces cerevisiae genes involved in various aspects of chromosome biology reveals that physical interactions fall into two well-represented classes distinguished by whether or not the individual proteins act coherently to carry out a common function.

TL;DR: The authors would like to thank members of the Hartwell laboratory, especially Eric Foss, member of the Seattle project, Jim Roberts, Andrew Murray, and an annonymous reviewer for helpful comments on the manuscript.

TL;DR: A review of the checkpoint's signal transduction pathway compares the role of these four classes of checkpoint proteins in humans and model organisms.

TL;DR: The identification of two adaptation-defective mutants that remain permanently arrested as large-budded cells when faced with an irreparable dsDNA break in a nonessential chromosome is reported.

TL;DR: It is shown that Ddc1 or Ddc2 fused to GFP localizes to a single subnuclear focus following an endonucleolytic break, which supports a model in which assembly of multiple checkpoint complexes at DNA damage sites stimulates checkpoint activation.