Dirk A. E. Dobbelaere

TL;DR: The PCR-RACE protocol, designed for the rapid amplification of cDNA ends, was successfully used to generate a full-length bovine IL4 (bIL4) cDNA clone from polyadenylated RNA isolated from concanavalin A-stimulated bovines lymph node cells.

TL;DR: A mouse monoclonal antibody (mAbD1) that neutralizes sporozoites of different stocks of the protozoan parasite Theileria parva has been used to localize and identify a sporozoite antigen.

TL;DR: The Tac antigen component of the bovine interleukin-2 receptor was expressed as a Cro-beta-galactosidase fusion protein in Escherichia coli and used to raise antibodies in rabbits to investigate the expression of Tac antigen in a variety of Theileria parva-infected cell lines and also in three Theilaria annulata- infected cell lines.

TL;DR: A mouse monoclonal antibody (mAbD1) that neutralizes sporozoites of different stocks of the protozoan parasite Theileria parva has been used to localize and identify a sporozoite antigen, which could be of relevance to the development of a broad spectrum vaccine against the cattle disease East Coast fever.

TL;DR: A complex pattern of Theileria SVSP mRNA expression is revealed, which depends on the parasite genotype, and the fact that a number of SVSPs contain functional nuclear localisation signals suggests that proteins released from the parasite could contribute to phenotypic changes of the host cell.