D
Dong-Myung Kim
Researcher at Chungnam National University
Publications - 254
Citations - 5562
Dong-Myung Kim is an academic researcher from Chungnam National University. The author has contributed to research in topics: Cell-free protein synthesis & Amino acid. The author has an hindex of 35, co-authored 246 publications receiving 5009 citations. Previous affiliations of Dong-Myung Kim include Genentech & Ajou University.
Papers
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Journal ArticleDOI
Regeneration of adenosine triphosphate from glycolytic intermediates for cell‐free protein synthesis
Dong-Myung Kim,James R. Swartz +1 more
TL;DR: It is reported that glucose-6-phosphate, an earlier intermediate of the glycolytic pathway, can be used for ATP regeneration and provide more stable maintenance of ATP concentration during protein synthesis.
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Prolonging cell‐free protein synthesis with a novel ATP regeneration system
Dong-Myung Kim,James R. Swartz +1 more
TL;DR: A new approach for the regeneration of adenosine triphosphate (ATP) during cell-free protein synthesis was developed to prolong the synthesis and also to avoid the accumulation of inorganic phosphate, demonstrated in a batch system derived from Escherichia coli.
Journal ArticleDOI
A highly efficient cell-free protein synthesis system from Escherichia coli.
TL;DR: A cell-free coupled transcription/translation system from Escherichia coli with the T7 phage RNA polymerase is modified, and it is found that the optimal concentrations of phosphoenolpyruvate and poly(ethylene glycol) are interdependent; higher concentrations of the former should be used at higher concentrationsof the latter.
Journal ArticleDOI
Prolonging Cell-Free Protein Synthesis by Selective Reagent Additions
Dong-Myung Kim,James R. Swartz +1 more
TL;DR: Through coordinated additions of PEP, arginine, cysteine, tryptophan, and magnesium, the final concentration of cell‐free synthesized CAT increased more than 4‐fold compared to a batch reaction.
Journal ArticleDOI
Simple procedures for the construction of a robust and cost-effective cell-free protein synthesis system
TL;DR: A simple method of preparing the cell extracts used for catalyzing cell-free protein synthesis reactions was developed, finding that the high-speed centrifugation, pre-incubation, and dialysis steps of the conventional procedures could be omitted without losing the translational activity of the resulting cell extract.