Dongneng Jiang

TL;DR: It is shown that amhy, a Y-specific duplicate of the anti-Müllerian hormone (amh) gene, induces male sex determination in Nile tilapia and the conserved roles of TGF-β signaling pathway in fish sex determination are highlighted.

TL;DR: The data demonstrate that targeted, heritable gene editing can be achieved in tilapia, providing a convenient and effective approach for generating loss-of-function mutants, and shows the utility of the CRISPR/Cas9 system for genetic engineering in non-model species like Tilapia and potentially in many other teleost species.

TL;DR: In the present study, randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) fingerprinting were used to screen pooled and individual DNA samples from XX, XY, and YY fish for sex-linked markers.

TL;DR: Results demonstrated that gsdf is a downstream gene of dmrt1, which probably inhibits estrogen production to trigger testicular differentiation, and inhibited estrogen production in a dose‐dependent manner in the presence of Sf1.

TL;DR: The sequence and expression patterns of most mi RNAs in tilapia are conserved in fishes, indicating the basic functions of vertebrate miRNAs might share a common evolutionary origin.