Hongjuan Shi

TL;DR: It is shown that amhy, a Y-specific duplicate of the anti-Müllerian hormone (amh) gene, induces male sex determination in Nile tilapia and the conserved roles of TGF-β signaling pathway in fish sex determination are highlighted.

TL;DR: The data demonstrate that targeted, heritable gene editing can be achieved in tilapia, providing a convenient and effective approach for generating loss-of-function mutants, and shows the utility of the CRISPR/Cas9 system for genetic engineering in non-model species like Tilapia and potentially in many other teleost species.

TL;DR: It is reported that TALENs can induce somatic mutations in Nile tilapia, an important species for worldwide aquaculture, with reliably high efficiency and is demonstrated to be an effective tool for targeted gene editing intilapia genome.

TL;DR: Foxl2 promotes ovarian development by upregulating Cyp19a1a expression and repressing male pathway gene expression and in vitro promoter analyses consistently demonstrated that Foxl2 could suppress the transcription of star1 in a dose-dependent manner.

TL;DR: Results demonstrated that gsdf is a downstream gene of dmrt1, which probably inhibits estrogen production to trigger testicular differentiation, and inhibited estrogen production in a dose‐dependent manner in the presence of Sf1.