The invention describes a screening method of a strain capable of producing acetoin and an acetoin production method based on a strain fermenting method, belonging to the technical field of the biological engineering. A soil sample of the strain is dried, suspended, diluted, coated, and then qualitatively analyzed and screened by a Voges-Proskauer(V-P) experiment, and then the screened strain is subject to physiological and biochemical and molecular biological identification, and a Bacillus amyloliquefaciens capable of producing the acetoin with high yield is obtained by using a high-performance liquid chromatography method. The invention also discloses an acetoin production method based on the strain, wherein the yield of the acetoin is 35g/L after fermenting lasts for 72 hours.

Screening method of strain capable of producing acetoin and acetoin production method based on strain fermenting method

The invention discloses an acid-producing klebsiella oxytoca MOW-02-05, a selection method and an application of the acid-producing Klebsiella oxytoca MOW-02-05, wherein the acid-producing klebsiella oxytoca MOW-02-05 is isolated from an intermittent anaerobic sludge reactor and can efficiently degrade dyes and organisms to produce hydrogen; and the klebsiella oxytoca MOW-02-05 is used for decolorizing azo dyes and biologically produce the hydrogen from organic carbon resources. The strain is identified as klebsiella oxytoca MOW-02-05 and is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 5237. The strain obtained by the invention can solve a problem caused by acidifying the reactor when a conventional anaerobic reactor treats azo dye wastewater, and is suitable for biological treatment of printing and dyeing dye wastewater.

Acid-producing klebsiella oxytoca MOW-02-05, selection method and application of acid-producing klebsiella oxytoca MOW-02-05

The invention relates to a method for producing 2,3-butanediol using improved strains of Raoultella planticola, and to novel mutant strains obtained by random mutagenesis from the bacterial species Raoultella planticola CECT843, that can be used in the industrial production of 2,3-butanediol from glycerol. The invention preferably relates to the Raoultella planticola strains designated IA1 and IIIA3 and deposited in the Spanish Type Culture Collection (CECT) under deposit number CECT8158 (corresponding to the strain designated IA1) and deposit number CECT8159 (corresponding to the strain designated IIIA3). The invention also relates to a method for producing 2,3-butanediol from glycerol by means of a biotechnological process using the novel strains of the invention.

Method for producing 2,3-butanediol using improved strains of raoultella planticola

Strain for producing 3-hydroxyl propionic acid at high yield and application thereof

The invention discloses a method for producing chiral pure (2S,3S)-2,3-butanediol, which is to express a (2S,3S)-2,3-butanediol dehydrogenase gene in Escherichia coli, and perform the conversion production of chiral pure (2S,3S)-2,3-butanediol by using the recombinant Escherichia coli full cell as a biocatalyst and diacetyl and glucose as a substrate. In the method disclosed by the invention, a culture medium required by the strain is simple and low in cost, and the production process and product separation are convenient. The highest yield of the produced chiral pure (2S,3S)-2,3-butanediol reaches over 26gL , the ee value is more than 99 percent, and the method has a promotion and application value and can create considerable economic benefit.

Method for producing chiral pure (2S,3S)-2,3-butanediol

The invention discloses a strain for generating 3-hydroxy butanone and the application thereof, which belong to the field of bioengineering technology. The stain is named as Paenibacillus polymyxa ME-J25 and is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.2505. The strain can be fermented to generate 3-hydroxy butanone with higher concentration, higher transformation efficiency and extremely low content of the reduced byproduct 2,3-butanediol of 3-hydroxy butanone, and is suitable for industrial production of 3-hydroxy butanone.

Bacterial strain for preparing 3-hydroxy butanone and uses thereof

The invention discloses a generating acid klebsiella and appliance, which is characterized by the following: setting the preserved number at CCTCC NO: M 207023; adopting ultraviolet and diethyl sulfate; composite-treating strain CCTCC NO: M 207023; seeding on selective solid medium with bromide of sodium and sodium bromate; culturing; sorting single bacterial colony; yeasting; preparing 2, 3-butylene glycol. This invention possesses simple operation, low cost, high efficiency and short period, which can increase receiving ratio of 2, 3-butylene glycol.

Acid-producing Klebsiella bacterium and uses thereof

The invention discloses a method for absorbing and separating 2, 3-butanediol in fermentation broth by applying hydrophobic zeolite. Hydrophobic zeolite is applied to absorbing 2, 3-butanediol from pretreated 2, 3-butanediol fermentation broth obtained from fungus fermentation, and anhydrous ethanol is applied to desorption and 2, 3-butanediol is obtained after removing ethanol in the desorption solution. The method of the invention has simple process, high separation efficiency, no impurities and low energy consumption and solves the bottleneck which restrains the current fermentation production process of 2, 3-butanediol, thus having comparatively good industrial application prospect.

Method for adsorptive separating 2, 3-butanediol from fermentation liquor by using hydrophobic zeolite

The invention relates to a transformation microbe containing a recombinant vector, wherein the recombinant vector contains protein expressed by a nucleotide sequence shown by a gene code SEQ ID NO.1. A method for preparing the transformation microbe comprises introducing the nucleotide sequence shown by the gene code SEQ ID NO.1 into a host. The transformation microbe containing the recombinant vector is applied to generating 3-hydracrylic acid. A plasmid has high stability in a host strain, and is quite suitable for generating a strain; the final concentration of a product has competitiveness; in addition, trace tetracycline can be added during fermentation so as to achieve the aim of inhibiting part hybrid bacteria, greatly reduce the phenomenon of easily dyeing the hybrid bacteria during industrialization, shorten the fermentation time and lay a good foundation for industrialization production of the 3-hydracrylic acid by a microbe fermentation method.

Transformation microbe generating 3-hydracrylic acid, construction method and application thereof

The invention discloses a method for preparing 2, 3- butanediol, belonging to biological chemical industry technique field. It comprises following steps: preparing culture medium, culturing seed and producing 2, 3- butanediol; adding exterior addition factor into fermentation culture medium before fermentation; or adding exterior addition factor during bacteria exponent growth period in 2, 3- butanediol producing process; said exterior addition factor is one or many of citrate, alpha- ketoglutaric acid, boletic acid and L- malic acid. The invention is characterized in that it can increase substrate conversion rate, increase concentration and production strength for target product 2, 3- butanediol, the operation is simple, needs no exterior labor and device, and production cost is low.

Du Jun

Papers

Bacterial strain for preparing 3-hydroxy butanone and uses thereof

Acid-producing Klebsiella bacterium and uses thereof

Method for adsorptive separating 2, 3-butanediol from fermentation liquor by using hydrophobic zeolite

Transformation microbe generating 3-hydracrylic acid, construction method and application thereof

Method for facilitating microbe synthesis of 2,3-butanediol by adding exogenous factor