The invention discloses an extraction method of microalga grease. The extraction method comprises the following steps of: (1) microalga cell collection: adjusting the pH value of a microalga culture solution to 11-12 at the later period of microalga cultivation, and collecting microalga cells in a centrifugal way; (2) microalga cell crushing: adding a C1-C6 short-chain alcohol solvent into microalga ooze obtained in the step (1), shaking and mixing the microalgae to be uniform, and then, carrying out cell breaking treatment on the microalgae; (3) grease extraction: adding one or more of organic solvents such as n-hexane and petroleum ether into a microalga cell breaking solution obtained in the step (2), and extracting the grease component in the cell breaking solution by using the organic solvents; and (4) solvent recovery: after the extraction reaction is ended, cooling the solvent to room temperature, standing for 10-30min, respectively extracting an organic solvent layer (oil phase) and a water layer (water phase), and recovering the organic solvents in the oil phase in a distillation way, and meanwhile, obtaining the microalga grease. The method has the advantages of simple operation process, short extraction time, low energy consumption, high microalga grease yield and the like and is suitable for directly extracting grease from alga ooze.

Extraction method of microalga grease

The invention provides a method for extracting DHA from cells of algae and fungi by breaking cell walls and relates to a method for extracting active ingredients from microorganisms. The method provided by the invention avoids the assistance from any organic solvent and chemical medicaments, breaks the walls of the cells of the algae and the fungi by using a physical method and extracts a grease product DHA (docosahexaenoic acid) from the cells and is suitable for large-scale production at low cost. The method comprises: after fermentation is finished, introducing fermentation liquor of microalgae or fungi to a separation system for separating and collecting the cells, adjusting the pH value of bacterial sludge with an acid to 2.0 to 4.0, keeping the temperature of the bacterial sludge between 10 and 20 DEG C, adding antioxidant into the bacterial sludge, and performing high-pressure homogenization and wall breaking by using a high-pressure homogenizer; and adding water into the bacterial sludge subjected to wall breaking, stirring the bacterial sludge, and separating feed liquor in a three-phase separator to obtain DHA grease. In the invention, physical wall breaking and the physical extracting method are used, the process is simple, the cell breaking is high efficient, the low-temperature treatment and antioxidant treatment of the bacterial sludge can effectively protect thebioactivities of the matters in the cells of the algae and the fungi, and the product is green, nontoxic and free from residues.

Method for extracting DHA from cells of algae and fungi by breaking cell walls

Disclosed is a method for extracting grease from fungi or alga cells rich in grease through physical wall-breaking. Upon ending of fermentation, fungal or algal fermentation broth undergoes separation performed by a separation system, cells are collected, and cell sludge is controlled. The cell sludge undergoes physical wall-breaking through a colloid mill or a high-pressure homogenizer. Secondary ball-milling wall-breaking and demulsification are performed through a ball mill. Finally, feed liquid undergoes separation through a three-phase separator to obtain grease rich in polyunsaturated fatty acids. The present invention uses a physical wall-breaking and extraction method, the process is simple, and the cell wall-breaking efficiency is high. Due to low-temperature and anti-oxidation treatment, active ingredients in the algal and fungal cells can be effectively protected. The product is green and non-toxic, and has no residue. The present invention is applicable to extraction of such grease as the DHA grease, the ARA grease, the EPA grease, the GLA grease, and the ALA grease, and is applicable to industrial production.

Method for extracting grease from fungi or alga through physical wall-breaking

Method for extracting schizochytrium aggregatum oil

The present invention discloses a method for controlling the amount of 3-chloro-1,2-propanediol or ester thereof during the refinement of oils and fats, , comprising degumming, bleaching, contacting the resultant bleached oil with water, isolating the water phase, drying the resultant oil phase, and deodorizing.

Method of controlling level of 3-chloro-1,2-propanediol or esters thereof in oils and fats

The invention discloses a process for extracting and refining fatty acid rich in DHA, from crypthecodinium cohnii, which comprises that first crypthecodinium cohnii fermentation liquor is flocculated,then solid and liquid are separated, walls are broken via alkali, and cells are mechanically broken, then the broken thalluses are extractsed via organic solvent, to obtain DHA rough oil, and the DHArough oil is degelatinized, alkali refined, decolorized, and deodorized to obtain DHA fine oil. The invention has simple operation and reduced extraction cost of DHA fat, which only uses organic solvent in operation, eliminates residue finally, and improves DHA fat quality by refining, while the DHA content in extractive is 40-50%, with DHA batch production support.

Technique for extracting and refining DHA enriched fatty acid from Crypthecodinium cohnii

The invention discloses a preparation method of arachidonic acid oil. The invention uses CCTCC NO: M207067 Mortierella alpine bacteria ME-AA01 to produce the arachidonic acid oil. The arachidonic acid oil applied with the method has advantages of high active components (containing 70%-83.1% archidonic acid residues), high quality and no EPA. The method effectively decreases the by-products in the fermentation process, improves the carbon turning arachidonic acid rate and the material using rate, and boasts low cost, easy operation and convenient industrialization.

Method of preparing arachidonic acid oil and fat

The invention discloses a generating acid klebsiella and appliance, which is characterized by the following: setting the preserved number at CCTCC NO: M 207023; adopting ultraviolet and diethyl sulfate; composite-treating strain CCTCC NO: M 207023; seeding on selective solid medium with bromide of sodium and sodium bromate; culturing; sorting single bacterial colony; yeasting; preparing 2, 3-butylene glycol. This invention possesses simple operation, low cost, high efficiency and short period, which can increase receiving ratio of 2, 3-butylene glycol.

Acid-producing Klebsiella bacterium and uses thereof

The invention discloses a mortierella alpine and the application thereof. Bacterial strains are conserved in China Center for Type Culture Collection (CCTCC) with a CCTCC NO. M 207067. The output of arachidonic acid produced by the bacterial strains upon fermentation can be up to 10g/l and the yield is up to 1.54g/l/d, thereby, the bacterial strains is applicable to industrial production of the arachidonic acid.

Mortierella alpina and uses thereof

The invention discloses a method for preparing 2, 3- butanediol, belonging to biological chemical industry technique field. It comprises following steps: preparing culture medium, culturing seed and producing 2, 3- butanediol; adding exterior addition factor into fermentation culture medium before fermentation; or adding exterior addition factor during bacteria exponent growth period in 2, 3- butanediol producing process; said exterior addition factor is one or many of citrate, alpha- ketoglutaric acid, boletic acid and L- malic acid. The invention is characterized in that it can increase substrate conversion rate, increase concentration and production strength for target product 2, 3- butanediol, the operation is simple, needs no exterior labor and device, and production cost is low.

Shuang Li

Papers

Technique for extracting and refining DHA enriched fatty acid from Crypthecodinium cohnii

Method of preparing arachidonic acid oil and fat

Acid-producing Klebsiella bacterium and uses thereof

Mortierella alpina and uses thereof

Method for facilitating microbe synthesis of 2,3-butanediol by adding exogenous factor