Dustin P. Patterson

TL;DR: The present study demonstrates incorporation of an enzyme, alcohol dehydrogenase D, with the highest internal loading for an active enzyme by any VLP described thus far, demonstrating that P22 holds potential for synthetic approaches to create nanoreactors, by design, using the power of highly evolved enzymes for chemical transformations.

TL;DR: A biomimetic approach for constructing densely packed and confined multienzyme systems through the co-encapsulation of 2 and 3 enzymes within a virus-like particle that perform a coupled cascade of reactions, creating a synthetic metabolon is described.

TL;DR: This work shows the encapsulation and protection of an active hydrogen-producing and oxygen-tolerant [NiFe]-hydrogenase, sequestered within the capsid of the bacteriophage P22 through directed self-assembly through probing the infrared spectroscopic signatures and catalytic activity of the engineered material.

TL;DR: The hierarchical bottom-up assembly of bacteriophage P22 virus-like particles (VLPs) that encapsulate the thermostable CelB glycosidase creating catalytically active nanoreactors producing P22 VLPs with a high packaging density of the tetrameric CelB, but without loss of enzyme activity.

TL;DR: Programmed encapsulation and sequestration of the conserved nucleoprotein from influenza on the interior of a VLP results in a vaccine that provides multistrain protection against 100 times lethal doses of influenza in an NP specific CD8+ T cell-dependent manner.