E
Edward C. Hulme
Researcher at National Institute for Medical Research
Publications - 101
Citations - 8882
Edward C. Hulme is an academic researcher from National Institute for Medical Research. The author has contributed to research in topics: Muscarinic acetylcholine receptor & Muscarinic acetylcholine receptor M1. The author has an hindex of 41, co-authored 101 publications receiving 8737 citations.
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Journal ArticleDOI
Muscarinic Receptor Subtypes
TL;DR: The aim of this review is to discuss the structure, function, and binding properties of the different muscarinic receptor species, attempting where possible to coordinate the diverse experimental data into a uniform picture.
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Pirenzepine distinguishes between different subclasses of muscarinic receptors
TL;DR: Binding studies using a new anti-muscarinic drug, pirenzepine, are used, in which heterogeneity of binding is found that correlates well with the pharmacological activity and cannot be taken as evidence for different receptor subtypes.
Journal Article
The binding of agonists to brain muscarinic receptors.
TL;DR: There is quantitative agreement between the agonist affinity constants for the two sites and parameters derived from the action of muscarinic agonists on smooth muscle, and a third (minor) population of "super-high" affinity agonist binding sites has been detected.
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Ligand binding assays at equilibrium: validation and interpretation.
Edward C. Hulme,Mike Trevethick +1 more
TL;DR: The focus of this review paper is factors affecting data interpretation in ligand binding assays under equilibrium conditions: buffer composition and the temperature at which the assay is conducted, and the impact these can have on affinity measurements and the understanding of drug interactions.
Journal ArticleDOI
C fragment of lipotropin has a high affinity for brain opiate receptors.
TL;DR: The C-terminal region of lipotropin has been found as an intact polypeptide in the pituitary gland and the C fragment is also released fromlipotropin by mild digestion with trypsin in vitro.