Elaine Holmes

TL;DR: A deeper understanding of the axes that physiologically connect the gut, liver, muscle, and brain are a prerequisite for optimizing therapeutic strategies to manipulate the gut microbiota to combat disease and improve health.

TL;DR: Metabonomics: understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data by using NMR data from Xenobiotica.

TL;DR: Metabonomics is a systems approach for studying in vivo metabolic profiles, which promises to provide information on drug toxicity, disease processes and gene function at several stages in the discovery-and-development process.

TL;DR: The main NMR spectroscopic applications in modern metabolic research are summarized, and detailed protocols for biofluid and tissue sample collection and preparation are provided, including the extraction of polar and lipophilic metabolites from tissues.

TL;DR: An overview of how the underlying philosophy of chemometrics is integrated throughout metabonomic studies is provided, including the tools applied for linear modeling, for example, Statistical Experimental Design (SED), Principal Component Analysis (PCA), Partial least-squares (PLS), Orthogonal-PLS, and dynamic extensions thereof.