Eleftherios T. Papoutsakis

TL;DR: A holistic, comparative and modular approach is taken in bringing together the large literature on genes, programs, mechanisms, processes and molecules involved in chemical stress or imparting tolerance, with an eye to future developments that engage novel genomic and systems-biology tools to create altered or semi-synthetic strains with superior tolerance characteristics for bioprocessing.

TL;DR: Analysis of the plasmid control strain 824(pSOS95del) revealed complex host-plasmid interactions relative to the wild-type strain, resulting in prolonged biphasic growth and metabolism, which suggests that groESL functions as a modulator of the CIRCE regulon.

TL;DR: Pathway engineering to combine established substrate-utilization programs, such as for cellulose, CO2/H2 or CO, with desirable metabolic programs could lead to modular design of strains suitable for many applications.

TL;DR: Clostridia are anaerobic, endospore-forming prokaryotes that include strains of importance to human and animal health and physiology, cellulose degradation, solvent production and bioremediation and indicate that a better understanding of the orchestration of the molecular events that underlie their unique physiology, capabilities and diversity will pay major dividends.

TL;DR: Solventogenic clostridia are strictly anaerobic, endospore forming bacteria that produce a large array of primary metabolites by anaerobically degrading simple and complex carbohydrates, including cellulose and hemicellulose, which suggest that they are promising organisms for biorefinery applications.