Emily J. Geddes

TL;DR: In this article, an analytical method for the quantification of organosulfates based upon ultra-performance liquid chromatography (UPLC) with negative electrospray ionization mass spectrometry (MS) with the aid of synthesized organosa-sulfate standards is presented.

TL;DR: This work provides tools for the facile discovery and development of high-accumulating compounds in E. coli, and a general blueprint for the conversion of Gram-positive-only compounds into broad-spectrum antibiotics.

TL;DR: Using recently developed predictive guidelines for compound accumulation in Escherichia coli, the antibiotic Ribocil C is converted from a compound lacking whole-cell activity against wild-type GN pathogens into a compound that accumulates to a high level in E. coli, is effective against Gram-negative clinical isolates, and has efficacy in mouse models of GN infection.

TL;DR: It is reported that other positively charged nitrogen functional groups, namely, N-alkyl guanid iniums and pyridiniums, can also facilitate compound uptake into Gram-negative bacteria.

TL;DR: The eNTRy rule as mentioned in this paper states that a compound is most likely to accumulate in E. coli if it has an ionizable Nitrogen, has low three-dimensionalality and is relatively Rigid.