Erick Sandoval

TL;DR: A plasmid-based internal control that produces a uniquely sized product and can be used to control for both reverse transcription or amplification steps without the risk of generating false-positive results is designed.

TL;DR: Interestingly, secondary infection was not significantly correlated with DHF/DSS, in contrast to previous studies in Southeast Asia, as evidenced by the analysis of this epidemic.

TL;DR: Given that saliva is a readily obtainable, noninvasive specimen, detection of DEN-specific salivary IgM should be considered a useful, cheaper diagnostic modality with similar sensitivity and specificity to IgM detection in serum.

TL;DR: Application of these simple PCR-based strain typing methods in a country endemic for dengue virus infections can help to characterize the transmission dynamics of this important emerging infectious disease problem and provide this information to local health authorities in a timely manner so that appropriate control measures can be implemented.

TL;DR: A simple, rapid, PCR-based subtyping method, restriction site-specific (RSS)-PCR, which was used to analyze dengue virus serotypes 2 and 3 and revealed that the RSS-PCR fingerprints reproducibly fell into 7 and 3 groups, respectively.