Erika Wolff

TL;DR: The results show that rpoB/Rif(r) is an accurate and easy to employ detection system, and offers the advantage of allowing analysis of mutations occurring on the chromosome rather than on an extrachromosomal element.

TL;DR: The crystal structure of the Escherichia coli MutL C‐terminal dimerization domain and the likelihood of its conservation among MutL homologs is reported and a model of how MutL utilizes its ATPase and DNA‐binding activities to mediate mismatch‐dependent activation of MutH endonuclease and UvrD helicase is proposed.

TL;DR: This analysis identifies several hot spots specific to Pol IV which are virtually absent from the normal spontaneous spectrum, indicating that Pol IV does not contribute significantly to mutations occurring during exponential growth in liquid culture.

TL;DR: A sequence analysis of the specificity of base substitution mutations generated in ndk and ndk mutS backgrounds as well as other experiments suggests that NDP kinase deficiency stimulates polymerase errors that lead to A:T --> G:C transitions and that the editing capacity of cells may be affected, leading to additional uncorrected mispairs and to A-T --> T:A transversions.

TL;DR: This genomic strategy offers an approach to identify novel mutator effects resulting from the multicopy cloning (MCC) of specific genes and therefore complementing the conventional gene inactivation approach to finding mutators.