Journal ArticleDOI
Use of the rpoB gene to determine the specificity of base substitution mutations on the Escherichia coli chromosome.
Lilit Garibyan,Tiffany T. Huang,Mandy Kim,Erika Wolff,Anh Nguyen,Theresa Nguyen,Amy Diep,Kaibin Hu,Ayuko Iverson,Hanjing Yang,Jeffrey H Miller +10 more
TLDR
The results show that rpoB/Rif(r) is an accurate and easy to employ detection system, and offers the advantage of allowing analysis of mutations occurring on the chromosome rather than on an extrachromosomal element.About:
This article is published in DNA Repair.The article was published on 2003-05-13. It has received 271 citations till now. The article focuses on the topics: rpoB & Ethyl methanesulfonate.read more
Citations
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Journal ArticleDOI
Rate and molecular spectrum of spontaneous mutations in the bacterium Escherichia coli as determined by whole-genome sequencing
TL;DR: Comparing results from the wild-type and MMR-defective strains may lead to a deeper understanding of factors that determine mutation rates and spectra, how these factors may differ among organisms, and how they may be shaped by environmental conditions.
Journal ArticleDOI
Evolution and Diversification of Lamprey Antigen Receptors: Evidence for Involvement of an AID-APOBEC Family Cytosine Deaminase
Igor B. Rogozin,Lakshminarayan M. Iyer,Lizhi Liang,Galina V. Glazko,Victoria G. Liston,Youri I. Pavlov,L. Aravind,Zeev Pancer +7 more
TL;DR: Lamprey lymphocytes express two putative deaminases of the AID-APOBEC family that may be involved in VLR diversification, as indicated by in vitro mutagenesis and recombination assays.
Journal ArticleDOI
Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors
Jordan L. Doman,Aditya Raguram,Aditya Raguram,Aditya Raguram,Gregory A. Newby,Gregory A. Newby,Gregory A. Newby,David R. Liu,David R. Liu,David R. Liu +9 more
TL;DR: The suite of CBEs characterized and engineered in this study collectively offer ~10–100-fold lower average Cas9-independent off-target DNA editing while maintaining robust on-target editing at most positions targetable by canonical CBEs, and thus are especially promising for applications in which off- target editing must be minimized.
Journal ArticleDOI
Determination of Antibiotic Hypersensitivity among 4,000 Single-Gene-Knockout Mutants of Escherichia coli
Cindy Tamae,Anne Liu,Katherine Kim,Daniel Sitz,Jeeyoon Hong,Elinne Becket,Ann Bui,Parrisa Solaimani,Katherine P. Tran,Hanjing Yang,Jeffrey H Miller +10 more
TL;DR: A number of double mutants are made with greatly increased sensitivity to ciprofloxacin, and these overcome the resistance generated by certain gyrA mutations, which provides innate resistance to antibiotics.
Journal ArticleDOI
The population genetics of antibiotic resistance: integrating molecular mechanisms and treatment contexts
TL;DR: This work focuses on the development of models that incorporate details of the molecular mechanisms of drug resistance and ecology into evolutionary models, and shows how these models can be helpful in predicting the dynamics of resistance evolution.
References
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Book
Introduction to Statistical Analysis
Wilfrid J. Dixon,Frank J. Massey +1 more
TL;DR: In this paper, the authors introduce statistical analysis and introduce the concept of statistical analysis in statistical analysis, and propose a framework for statistical analysis for the analysis of statistical data in the literature.
Book
A short course in bacterial genetics : a laboratory manual and handbook for Escherichia coli and related bacteria
TL;DR: Experiments in Molecular Genetics as mentioned in this paper is a spiral bound manual for those doing genetic or recombinant DNA work with E. coli or similar organisms with step-by-step protocols and clear diagrams that demonstrate major concep
Journal ArticleDOI
An improved bacterial test system for the detection and classification of mutagens and carcinogens
TL;DR: A test that compares mutagenic killing in deep rough strains with and without DNA excision repair, and a test using forward mutagenesis in a deep rough strain lacking excison repair are described.
Journal ArticleDOI
Structural Mechanism for Rifampicin Inhibition of Bacterial RNA Polymerase
Elizabeth A. Campbell,Nataliya Korzheva,Arkady Mustaev,Katsuhiko S. Murakami,Satish K. Nair,Alex Goldfarb,Seth A. Darst +6 more
TL;DR: The crystal structure of Thermus aquaticus core RNAP complexed with Rif explains the effects of Rif on RNAP function and indicates that the inhibitor acts by directly blocking the path of the elongating RNA when the transcript becomes 2 to 3 nt in length.
Journal ArticleDOI
Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis
Amalio Telenti,P Imboden,F Marchesi,Lukas Matter,K Schopfer,Thomas Bodmer,Douglas B. Lowrie,M.J Colston,Stewart T. Cole +8 more
TL;DR: Substitution of a limited number of highly conserved aminoacids encoded by the rpoB gene appears to be the molecular mechanism responsible for "single step" high-level resistance to rifampicin in M tuberculosis, a marker of multidrug-resistant tuberculosis.
Related Papers (5)
Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance.
Ding Jun Jin,Carol A. Gross +1 more