Estrella Duque

TL;DR: The number of efflux pump operons has been found to correlate with the degree of solvent tolerance in different P. putida strains, and the operation of these efflux pumps seems to be coupled to the proton motive force via the TonB system, although the intimate mechanism of energy transfer remains elusive.

TL;DR: Pseudomonas putida DOT-T1 was isolated after enrichment on minimal medium and used the toluene-4-monooxygenase pathway to catabolyze toluenes, and the catabolic potential was expanded to include m- and p-xylene and related hydrocarbons by transfer of the TOL plasmid pWW0-Km.

TL;DR: Mutants unable to carry out cis → trans isomerization of unsaturated lipids, that exhibit altered cell envelopes because of the lack of the OprL protein, or that are unable to exclude toluene from cell membranes are hypersensitive to toluenes.

TL;DR: The identification and characterization in this strain of a third efflux pump, named TtgGHI, is reported, which was shown to be unable to survive sudden 0.3% (vol/vol) toluene shocks regardless of the preculture conditions.

TL;DR: The basic mechanisms underlying solvent tolerance in Pseudomonas putida DOT-T1E are efflux pumps that remove the solvent from bacterial cell membranes and the mutant was unable to remove 1,2,4-[14C]trichlorobenzene from the cell membranes when grown on Luria-Bertani medium but was able to remove the aromatic compound when pregrown on LB medium with toluene supplied via the gas phase.