E
Eugenia Spanopoulou
Researcher at Icahn School of Medicine at Mount Sinai
Publications - 22
Citations - 2348
Eugenia Spanopoulou is an academic researcher from Icahn School of Medicine at Mount Sinai. The author has contributed to research in topics: V(D)J recombination & RAG2. The author has an hindex of 19, co-authored 22 publications receiving 2313 citations. Previous affiliations of Eugenia Spanopoulou include Howard Hughes Medical Institute & Rockefeller University.
Papers
More filters
Journal ArticleDOI
Functional immunoglobulin transgenes guide ordered B-cell differentiation in Rag-1-deficient mice.
Eugenia Spanopoulou,Christopher A. J. Roman,Lynn M. Corcoran,Mark S. Schlissel,Daniel P. Silver,David Nemazee,Michel C. Nussenzweig,Susan A. Shinton,Richard R. Hardy,David Baltimore +9 more
TL;DR: This experimental system demonstrates the competence of the mu HC and kappa LC to direct and regulate the sequential stages of B-cell differentiation, defines the time at which negative selection of self-reactive B cells occurs, and shows that elimination of these cells occurs equally well in the absence of Rag-1 as in its presence.
Journal ArticleDOI
Partial V(D)J Recombination Activity Leads to Omenn Syndrome
Anna Villa,Sandro Santagata,Fabio Bozzi,Silvia Giliani,Annalisa Frattini,Luisa Imberti,Luisa Benerini Gatta,Hans D. Ochs,Klaus Schwarz,Luigi D. Notarangelo,Paolo Vezzoni,Eugenia Spanopoulou +11 more
TL;DR: It is reported here that patients with Omenn syndrome, a severe immunodeficiency characterized by the presence of activated, anergic, oligoclonal T cells, hypereosinophilia, and high IgE levels, bear missense mutations in either the Rag-1 or Rag-2 genes that result in partial activity of the two proteins.
Journal ArticleDOI
The Homeodomain Region of Rag-1 Reveals the Parallel Mechanisms of Bacterial and V(D)J Recombination
Eugenia Spanopoulou,Florina Zaitseva,Fu-Hou Wang,Sandro Santagata,David Baltimore,George Panayotou +5 more
TL;DR: Once the Rag-1/Rag-2 complex is engaged on the DNA, subsequent cleavage is directed by the heptamer sequence, which remarkably parallels mechanisms that mediate transposition in bacteria and nematodes.
Journal ArticleDOI
RAG1 mediates signal sequence recognition and recruitment of RAG2 in V(D)J recombination.
Michael J. Difilippantonio,Mcmahan Catherine J,Quinn M. Eastman,Eugenia Spanopoulou,David G. Schatz +4 more
TL;DR: An in vivo one-hybrid DNA binding assay is used to demonstrate that RAG1, in the absence of RAG2, can mediate signal recognition via the nonamer, with the heptamer acting to enhance its binding.
Journal ArticleDOI
Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination
TL;DR: The results indicate that the N-terminal one-third of RAG-1, including a zinc-finger-like domain, and an acidic domain of R AG-2 are dispensable for activating V(D)J recombination in a fibroblast, although they contribute quantitatively.