E
Evgenia Platonova
Researcher at Randall Division of Cell and Molecular Biophysics
Publications - 7
Citations - 817
Evgenia Platonova is an academic researcher from Randall Division of Cell and Molecular Biophysics. The author has contributed to research in topics: Photoactivated localization microscopy & Microscopy. The author has an hindex of 6, co-authored 7 publications receiving 707 citations. Previous affiliations of Evgenia Platonova include ETH Zurich.
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A simple, versatile method for GFP-based super-resolution microscopy via nanobodies
TL;DR: This work developed a method to use any GFP-tagged construct in single-molecule super-resolution microscopy by targeting GFP with small, high-affinity antibodies coupled to organic dyes and achieved nanometer spatial resolution and minimal linkage error when analyzing microtubules, living neurons and yeast cells.
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mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities
Ann L. McEvoy,Hiofan Hoi,Mark Bates,Evgenia Platonova,Paula J. Cranfill,Paula J. Cranfill,Michelle A. Baird,Michelle A. Baird,Michael W. Davidson,Michael W. Davidson,Helge Ewers,Jan Liphardt,Robert E. Campbell +12 more
TL;DR: An engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super-resolution imaging modalities, specifically, widefield and confocal microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy.
Journal ArticleDOI
Single-molecule microscopy of molecules tagged with GFP or RFP derivatives in mammalian cells using nanobody binders
Evgenia Platonova,Christian M. Winterflood,Alexander Junemann,David Albrecht,Jan Faix,Helge Ewers,Helge Ewers,Helge Ewers +7 more
TL;DR: This work illustrates in detail how to use small, high affinity nanobody binders against GFP and RFP family proteins for highly generic labeling of fusion constructs with bright organic dyes and provides detailed protocols and examples for their application in superresolution imaging and single particle tracking.
Journal ArticleDOI
A Simple Method for GFP- and RFP-based Dual Color Single-Molecule Localization Microscopy
Evgenia Platonova,Evgenia Platonova,Christian M. Winterflood,Christian M. Winterflood,Helge Ewers,Helge Ewers,Helge Ewers +6 more
TL;DR: This work provides a generic approach for high-quality simultaneous two-color single-molecule localization microscopy imaging of any combination of GFP- and RFP-tagged proteins with the use of nanobodies with only minimal technical requirements.
Journal ArticleDOI
Single event visualization of unconventional secretion of FGF2
Eleni Dimou,Katia Cosentino,Evgenia Platonova,Uris Ros,Mohsen Sadeghi,Purba Kashyap,Taxiarchis Katsinelos,Sabine Wegehingel,Frank Noé,Ana J. García-Sáez,Helge Ewers,Helge Ewers,Walter Nickel +12 more
TL;DR: This work directly visualized individual FGF2 membrane translocation events at the plasma membrane using live cell TIRF microscopy and directly demonstrated FGF 2 oligomers at the inner leaflet of living cells with a FGF1 dimer being the most prominent species.