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Paula J. Cranfill
Researcher at Florida State University
Publications - 10
Citations - 3449
Paula J. Cranfill is an academic researcher from Florida State University. The author has contributed to research in topics: Green fluorescent protein & Fluorescence. The author has an hindex of 10, co-authored 10 publications receiving 2896 citations. Previous affiliations of Paula J. Cranfill include University of California, Berkeley & Vanderbilt University.
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Journal ArticleDOI
A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum.
Nathan C. Shaner,Gerard G. Lambert,Andrew Chammas,Yuhui Ni,Paula J. Cranfill,Michelle A. Baird,Brittney R. Sell,John R. Allen,Richard O. Day,Maria Israelsson,Michael W. Davidson,Jiwu Wang +11 more
TL;DR: In this article, a monomeric yellow green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum, was described.
Journal ArticleDOI
Improving FRET dynamic range with bright green and red fluorescent proteins
Amy J. Lam,François St-Pierre,Yiyang Gong,Yiyang Gong,Jesse D. Marshall,Jesse D. Marshall,Paula J. Cranfill,Michelle A. Baird,Michael R. McKeown,Jörg Wiedenmann,Michael W. Davidson,Mark J. Schnitzer,Mark J. Schnitzer,Roger Y. Tsien,Michael Z. Lin +14 more
TL;DR: Replacement of CFP and YFP with these two proteins in reporters of kinase activity, small GTPase activity and transmembrane voltage significantly improves photostability, FRET dynamic range and emission ratio changes and enhances detection of transient biochemical events.
Journal ArticleDOI
Quantitative assessment of fluorescent proteins
Paula J. Cranfill,Paula J. Cranfill,Paula J. Cranfill,Brittney R. Sell,Michelle A. Baird,John R. Allen,Zeno Lavagnino,Zeno Lavagnino,H. Martijn de Gruiter,Gert-Jan Kremers,Michael W. Davidson,Alessandro Ustione,Alessandro Ustione,David W. Piston,David W. Piston +14 more
TL;DR: To guide decisions about which FP is right for a given application, quantitatively characterized the brightness, photostability, pH stability and monomeric properties of more than 40 FPs to enable straightforward and direct comparison between them.
Journal ArticleDOI
An Enhanced Monomeric Blue Fluorescent Protein with the High Chemical Stability of the Chromophore
TL;DR: The chemical structure of the degraded product of the blue mTagBFP-like chromophore is determined and an improved variant, named m tagBFP2, is developed, which exhibits 2-fold greater chemical stability and substantially higher brightness in live cells than m TagBFP.
Journal ArticleDOI
An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching
Michele L. Markwardt,Gert-Jan Kremers,Catherine A. Kraft,Krishanu Ray,Paula J. Cranfill,Korey A. Wilson,Richard O. Day,Rebekka M. Wachter,Michael W. Davidson,Megan A. Rizzo +9 more
TL;DR: MCerulean3 is a bright, photostable cyan fluorescent protein which possesses several characteristics that are highly desirable for FRET experiments and fits to a single exponential time constant, making mCerULEan3 a suitable choice for fluorescence lifetime microscopy experiments.