F. J. de Bruijn

TL;DR: The state-of-the-art methods described in MMEM-II have not only been provided by experts in the field, but in most cases by the laboratories that actually first developed and applied the methods, thus providing the MM EM-II user with unique first-hand tips and insight.

TL;DR: Isolates which had been postulated to beclosely related bymultilocus enzyme electrophoresis alsorevealed similar REP andERICPCR patterns, suggesting that theREP andERicPCR sequences are closely related.

TL;DR: The more than 13,400 plant ESTs that are currently available provide a new resource that will facilitate progress in many areas of plant biology and are illustrated by a description of the results obtained from analysis of 1500 Arabidopsis ESTs from a cDNA library prepared from equal portions of poly(A+) mRNA from etiolated seedlings, roots, leaves, and flowering inflorescence.

TL;DR: The rep-PCR technique appears to be a rapid, simple, and reproducible method to identify and classify Xanthomonas and Pseudomonas strains, and it may be a useful diagnostic tool for these important plant pathogens.

TL;DR: The properties of transposon Tn5 that render it useful for in vivo mutagenesis of cloned DNA sequences are reviewed and a methods section is included which outlines precisely how to carry out transposition frequency, insertional specificity, polarity and stability of Tn 5 insertion mutations.