S‐Scheme Photocatalytic Systems

Mesoporous black TiO2/MoS2/Cu2S hierarchical tandem heterojunctions toward optimized photothermal-photocatalytic fuel production

Chromatographic stationary phase is the core of high-performance liquid chromatography (HPLC) system. Thereinto, the development of novel stationary phases with high separation efficiency and excellent selectivity has always been the focus of the study. Mixed-mode stationary phases have been proved to be with the merits of high separation selectivity, remarkably high loading capacity, and high separation efficiency. In this study, the recently reported particle-based mixed-mode stationary phases are summarized, especially for those reported in the recent 5 years. These stationary phases are classified based on the HPLC modes, emphasizing the preparation methods and further applications. Furthermore, the recently reported monolithic-based mixed-mode stationary phases for HPLC are also included.

Mixed-mode chromatographic stationary phases: Recent advancements and its applications for high-performance liquid chromatography

Enzymes are of great importance in the industry due to their substrate and product specificity, moderate reaction conditions, minimal by-product formation and high yield. They are important ingredients in several products and production processes. Up to 30% of the total production cost of enzymes is attributed to the raw materials costs. The food industry expels copious amounts of processing waste annually, which is mostly lignocellulosic in nature. Upon proper treatment, lignocellulose can replace conventional carbon sources in media preparations for industrial microbial processes, such as enzyme production. However, wild strains of microorganisms that produce industrially important enzymes show low yield and cannot thrive on artificial substrates. The application of recombinant DNA technology and metabolic engineering has enabled researchers to develop superior strains that can not only withstand harsh environmental conditions within a bioreactor but also ensure timely delivery of optimal results. This article gives an overview of the current complications encountered in enzyme production and how accumulating food processing waste can emerge as an environment-friendly and economically feasible solution for a choice of raw material. It also substantiates the latest techniques that have emerged in enzyme purification and recovery over the past four years.

https://www.mdpi.com/2306-5354/3/4/30/pdf

Microbial Enzyme Production Using Lignocellulosic Food Industry Wastes as Feedstock: A Review

Accelerated photocatalytic degradation of tetracycline hydrochloride over CuAl2O4/g-C3N4 p-n heterojunctions under visible light irradiation

Engineering Surface N-Vacancy Defects of Ultrathin Mesoporous Carbon Nitride Nanosheets as Efficient Visible-Light-Driven Photocatalysts

Preparation of a novel dual-function strong cation exchange/hydrophobic interaction chromatography stationary phase for protein separation.

Engineering surface oxygen vacancy of mesoporous CeO2 nanosheets assembled microspheres for boosting solar-driven photocatalytic performance

In this study, 3-diethylamino-1-propyne was covalently bonded to the azide-silica by a click reaction to obtain a novel dual-function mixed-mode chromatography stationary phase for protein separation with a ligand containing tertiary amine and two ethyl groups capable of electrostatic and hydrophobic interaction functionalities, which can display hydrophobic interaction chromatography character in a high-salt-concentration mobile phase and weak anion exchange character in a low-salt-concentration mobile phase employed for protein separation. As a result, it can be employed to separate proteins with weak anion exchange and hydrophobic interaction modes, respectively. The resolution and selectivity of the stationary phase were evaluated in both hydrophobic interaction and ion exchange modes with standard proteins, respectively, which can be comparable to that of conventional weak anion exchange and hydrophobic interaction chromatography columns. Therefore, the synthesized weak anion exchange/hydrophobic interaction dual-function mixed-mode chromatography column can be used to replace two corresponding conventional weak anion exchange and hydrophobic interaction chromatography columns to separate proteins. Based on this mixed-mode chromatography stationary phase, a new off-line two-dimensional liquid chromatography technology using only a single dual-function mixed-mode chromatography column was developed. Nine kinds of tested proteins can be separated completely using the developed method within 2.0 h.

Preparation of a weak anion exchange/hydrophobic interaction dual-function mixed-mode chromatography stationary phase for protein separation using click chemistry

Fan Yang

Papers

Engineering Surface N-Vacancy Defects of Ultrathin Mesoporous Carbon Nitride Nanosheets as Efficient Visible-Light-Driven Photocatalysts

Preparation of a novel dual-function strong cation exchange/hydrophobic interaction chromatography stationary phase for protein separation.

Engineering surface oxygen vacancy of mesoporous CeO2 nanosheets assembled microspheres for boosting solar-driven photocatalytic performance

Preparation of a weak anion exchange/hydrophobic interaction dual-function mixed-mode chromatography stationary phase for protein separation using click chemistry

Preparation of monodisperse large-porous silica microspheres with polymer microspheres as the templates for protein separation