Fanyu Meng

TL;DR: A statistical model is described that shows that the database search specificity of the direct fragmentation of protein ions in a mass spectrometer requires only three of four fragment ions to match for a 99.8% probability of being correct in a database of 5,000 protein forms.

TL;DR: The ALS-PAGE/RPLC approach to proteome processing ameliorates the "front end" problem that accompanies direct analysis of whole proteins and assists the future realization of protein identification with 100% sequence coverage in a high-throughput format.

TL;DR: A Web-based software and database suite called ProSight PTM constructed for large-scale proteome projects involving direct fragmentation of intact protein ions, which is described as the first search engine and Web environment for identification of intact proteins.

TL;DR: FTMS will continue its expansion into more laboratories in part because of its ability to detect and deconvolute the regulatory mechanisms of biology written in the language of PTMs.

TL;DR: Synthetic histones generated by this method are fully functional, as evidenced by their self-assembly into a higher order H3/H4 heterotetramer, their deposition into nucleosomes by human ISWI-containing RSF (Remodeling and Spacing Factor), and by enzymatic modification by human Sirt1 deacetylase and G9a methyltransferase.