Felipe C. Cabello

TL;DR: Global efforts are needed to promote more judicious use of prophylactic antibiotics in aquaculture as accumulating evidence indicates that unrestricted use is detrimental to fish, terrestrial animals, and human health and the environment.

TL;DR: The World Wildlife Fund is facilitating a dialogue on impacts of salmon Aquaculture to establish the state of knowledge in seven subject areas associated with the industry: benthic impacts, nutrient loading, escapees, chemical inputs, diseases, feeds and social issues and to establish international standards for salmon aquaculture practices.

TL;DR: Excessive use of antimicrobials in aquaculture can potentially negatively impact animal and human health as well as the aquatic environment and should be better assessed and regulated.

TL;DR: A procedure for precise assembly of linear DNA constructs as long as 20 kb, using Pfu polymerase mix, which has a proofreading activity, is proposed, offering an additional improvement on the combination of long PCR and overlap extension PCR.

TL;DR: Using a combined strategy of gene disruption and genetic complementation targeting rpoN and rpoS in Borrelia burgdorferi strain 297, a regulatory network is described that governs the expression of RpoS, which, in turn, governs theexpression of two important membrane lipoproteins, outer surface protein C and decorin-binding protein A, and likely other proteins of B. burgdorFERi.