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Anton V. Bryksin
Researcher at Georgia Institute of Technology
Publications - 36
Citations - 1991
Anton V. Bryksin is an academic researcher from Georgia Institute of Technology. The author has contributed to research in topics: Borrelia burgdorferi & Plasmid. The author has an hindex of 17, co-authored 33 publications receiving 1585 citations. Previous affiliations of Anton V. Bryksin include Parker H. Petit Institute for Bioengineering & Bioscience & New York Medical College.
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Journal ArticleDOI
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids
TL;DR: A straightforward, efficient, and reliable way to clone an insert of choice into a plasmid of choice without restriction endonucleases or T4 DNA ligase is described.
Journal ArticleDOI
Construction of long DNA molecules using long PCR‐based fusion of several fragments simultaneously
Nikolai A. Shevchuk,Anton V. Bryksin,Yevgeniya A. Nusinovich,Felipe C. Cabello,Margaret Sutherland,Stephan Ladisch +5 more
TL;DR: A procedure for precise assembly of linear DNA constructs as long as 20 kb, using Pfu polymerase mix, which has a proofreading activity, is proposed, offering an additional improvement on the combination of long PCR and overlap extension PCR.
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High-throughput in vivo screen of functional mRNA delivery identifies nanoparticles for endothelial cell gene editing.
Cory D. Sago,Melissa P. Lokugamage,Kalina Paunovska,Daryll Vanover,Christopher M. Monaco,Nirav N. Shah,Marielena Gamboa Castro,Shannon E. Anderson,Tobi G. Rudoltz,Gwyneth N. Lando,Pooja M. Tiwari,Jonathan L. Kirschman,Nick J. Willett,Young C. Jang,Philip J. Santangelo,Anton V. Bryksin,James E. Dahlman +16 more
TL;DR: A high-throughput method to measure how >100 nanoparticles delivered mRNA that was translated into functional protein in vivo and identify 7C2 and 7C3, two LNPs that efficiently deliver siRNA, single-guide RNA (sgRNA), and mRNA to endothelial cells.
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A Direct Comparison of in Vitro and in Vivo Nucleic Acid Delivery Mediated by Hundreds of Nanoparticles Reveals a Weak Correlation.
Kalina Paunovska,Cory D. Sago,Christopher M. Monaco,William H. Hudson,Marielena Gamboa Castro,Tobi G. Rudoltz,Sujay Kalathoor,Daryll Vanover,Philip J. Santangelo,Rafi Ahmed,Anton V. Bryksin,James E. Dahlman +11 more
TL;DR: Using high throughput LNP barcoding, data demonstrate that barcoded LNPs can elucidate fundamental questions about in vivo nanoparticle delivery and does LNP delivery change within the microenvironment of a tissue.
Journal ArticleDOI
Rational Design of a Plasmid Origin That Replicates Efficiently in Both Gram-Positive and Gram-Negative Bacteria
TL;DR: The results demonstrate the efficiency of pBAV1K-T5 replication in different bacterial species, thereby facilitating the study of proteins that don't fold well in E. coli and pathogens not amenable to existing genetic tools.