Félix Recillas-Targa

TL;DR: It is shown that the chicken insulator can protect a stably integrated gene, which is otherwise subject to great variability of expression, from chromatin-mediated repression in cell culture, and proposed a model for the insulator's ability to protect against extinction in the transformed cell lines and to function as a chromatin boundary for the chicken beta-globin locus in normal erythroid cells.

TL;DR: It is shown here that a 250-bp “core” element from within 5′HS4 is sufficient to confer protection against silencing of transgenes caused by CPE, and compared with those of other CTCF-binding enhancer-blocking elements suggests that CPE protection is associated with maintenance of a high level of histone acetylation near the insulator.

TL;DR: There is a very strong constitutive focus of hyperacetylation at the 5′ insulator element separating the globin locus from the folate receptor region, which suggests that this insulators element may harbor a high concentration of histone acetylases.

TL;DR: DNA sequence elements that can serve in some cases as barriers to protect a gene against the encroachment of adjacent inactive condensed chromatin also can act as blocking elements to protect against the activating influence of distal enhancers associated with other genes.

TL;DR: Par-CLIP-seq analyses indicate that CTCF binds a multitude of transcripts genome-wide as well as to Wrap53 RNA, which may bear directly on its role in establishing higher-order chromatin structures in vivo.