F
Florian A. Steiner
Researcher at University of Geneva
Publications - 28
Citations - 1352
Florian A. Steiner is an academic researcher from University of Geneva. The author has contributed to research in topics: Chromatin & Histone. The author has an hindex of 10, co-authored 27 publications receiving 1210 citations. Previous affiliations of Florian A. Steiner include Fred Hutchinson Cancer Research Center & Howard Hughes Medical Institute.
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Journal ArticleDOI
Secondary siRNAs Result from Unprimed RNA Synthesis and Form a Distinct Class
TL;DR: Cloned secondary siRNAs from transgenic C. elegans lines expressing a single 22-nucleotide primary siRNA indicated that non–RISC (RNA-induced silencing complex)–cleaved mRNAs are substrates for secondary siRNA production, infer that RdRPs perform unprimed RNA synthesis.
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Cloning and expression of new microRNAs from zebrafish
Wigard P. Kloosterman,Florian A. Steiner,Eugene Berezikov,Ewart de Bruijn,Jose van de Belt,Mark Verheul,Edwin Cuppen,Ronald H.A. Plasterk +7 more
TL;DR: The cloning and expression analysis indicates that most abundant and conserved miRNAs in zebrafish are now known, and it is found that most mi RNAs were expressed during later stages of development.
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Cell-type-specific nuclei purification from whole animals for genome-wide expression and chromatin profiling
TL;DR: A method for purifying nuclei from specific cell types of animal models that allows simultaneous determination of both expression and chromatin profiles is described, based on in vivo biotin-labeling of the nuclear envelope and subsequent affinity purification of nuclei.
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Holocentromeres are dispersed point centromeres localized at transcription factor hotspots
TL;DR: The results show that the point centromere is the basic unit of holocentric organization in support of the classical polycentric model for holocentromeres, and provide a mechanistic basis for understanding how centromeric chromatin might be maintained.
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Structural features of small RNA precursors determine Argonaute loading in Caenorhabditis elegans.
Florian A. Steiner,Suzanne W. Hoogstrate,Kristy L. Okihara,Karen L. Thijssen,René F. Ketting,Ronald H.A. Plasterk,Titia Sijen +6 more
TL;DR: In C. elegans, DCR-1 is required for the maturation of both short interfering RNAs and microRNAs, which are subsequently loaded into different Argonaute proteins to mediate silencing via distinct mechanisms, and structural features that direct the small RNAs into the RNA interference (RNAi) pathway or the miRNA-processing pathway are shown.