F
Florian Nachon
Researcher at Centre national de la recherche scientifique
Publications - 140
Citations - 5966
Florian Nachon is an academic researcher from Centre national de la recherche scientifique. The author has contributed to research in topics: Butyrylcholinesterase & Acetylcholinesterase. The author has an hindex of 40, co-authored 131 publications receiving 5016 citations. Previous affiliations of Florian Nachon include University of Nebraska Medical Center & Eppley Institute for Research in Cancer and Allied Diseases.
Papers
More filters
Journal ArticleDOI
Crystal structure of human butyrylcholinesterase and of its complexes with substrate and products.
TL;DR: The crystal structures of several recombinant truncated human BChE complexes and conjugates are reported and a description for mechanistically relevant non-productive substrate and product binding is provided and is similar to a previously published theoretical model of this enzyme.
Journal ArticleDOI
Reactivators of Acetylcholinesterase Inhibited by Organophosphorus Nerve Agents
Guillaume Mercey,Guillaume Mercey,Tristan Verdelet,Tristan Verdelet,Julien Renou,Julien Renou,Maria Kliachyna,Rachid Baati,Florian Nachon,Ludovic Jean,Ludovic Jean,Pierre-Yves Renard,Pierre-Yves Renard,Pierre-Yves Renard +13 more
TL;DR: This Account summarizes recent strategies for the development of AChE reactivators capable of crossing the blood-brain barrier (BBB) efficiently and the use of nanoparticulate transport and inhibition of P-glycoprotein efflux pumps improves BBB transport of these A cholinesterase reactsivators.
Journal ArticleDOI
Crystal structures of human cholinesterases in complex with huprine W and tacrine: elements of specificity for anti-Alzheimer's drugs targeting acetyl- and butyryl-cholinesterase.
Florian Nachon,Eugénie Carletti,Cyril Ronco,Marie Trovaslet,Yvain Nicolet,Ludovic Jean,Pierre-Yves Renard +6 more
TL;DR: Huprine W in hAChE and tacrine in hBChE reside in strikingly similar positions highlighting the conservation of key interactions, namely, π-π/cation-π interactions with Trp86 (Trp82), and hydrogen bonding with the main chain carbonyl of the catalytic histidine residue.
Journal ArticleDOI
Comparison of the Binding of Reversible Inhibitors to Human Butyrylcholinesterase and Acetylcholinesterase: A Crystallographic, Kinetic and Calorimetric Study.
Terrone L. Rosenberry,Xavier Brazzolotto,Ian R. Macdonald,Marielle Wandhammer,Marie Trovaslet-Leroy,Sultan Darvesh,Florian Nachon +6 more
TL;DR: Crystal structures of human BChE in complex with five reversible ligands, namely, decamethonium, thioflavin T, propidium, huprine, and ethopropazine are solved and this new information allows us to define the binding mode of various ligand families and will be of importance in designing specific reversible liganded of AChE that behave as inhibitors or reactivators.
Journal ArticleDOI
Progress in the development of enzyme-based nerve agent bioscavengers ☆
TL;DR: Pre- or post-exposure administration of bioscavengers, enzymes that neutralize and detoxify organophosphorus molecules, is one of the major developments of new medical counter-measures and research efforts focus on improving its catalytic efficiency toward the most toxic isomers of nerve agents by means of directed evolution-based strategies.