Francesc Gòdia

TL;DR: Among the different compounds studied, galactose and glutamate provide the best results, allowing support of cell growth with an optimal balance between nutrient uptake and cell requirements and the generation of minimal quantities of lactate and ammonium.

TL;DR: The state-of-the-art of VLP production systems and the newest generation of V LP-based vaccines now available are reviewed.

TL;DR: A CHO cell line producing t-PA was cultured using glutamate and glucose or galactose to decrease the formation of metabolic end-products and therefore improving the process, suggesting that the lactate produced is not used in the oxidative metabolism through the TCA cycle.

TL;DR: A strategy for fed-batch cultivation of t-PA producing recombinant CHO cells is presented, based on the substitution of glucose and glutamine for slowly metabolized nutrients and in a rational design of the medium, that altogether increased viability, longevity and t- PA production when compared with a reference batch culture.

TL;DR: The results obtained with the two more advanced bioreactors in the Micro Ecological Life Support System Alternative (MELISSA) loop are described more specifically and their individual operation for long duration runs has been achieved and characterized, and their interconnected operation at pilot scale is reported.