Andrés Illanes

TL;DR: In the present work, recent advances in biocatalysis are reviewed and powerful tools to improve biotransformation and to synthesize new products are reviewed.

TL;DR: Models are proposed to consider the presence of substrate and products on biocatalyst stability and thus describe properly bioreactor performance, a key factor for using enzymes in organic synthesis where nonaqueous media are mandatory or at least highly desirable.

TL;DR: A strategy for fed-batch cultivation of t-PA producing recombinant CHO cells is presented, based on the substitution of glucose and glutamine for slowly metabolized nutrients and in a rational design of the medium, that altogether increased viability, longevity and t- PA production when compared with a reference batch culture.

TL;DR: The effect of glutamine replacement by glutamate and the balance between glutamate and glucose metabolism on the redistribution of t‐PA‐producing recombinant CHO cells metabolism is studied in a series of glucose shift down and shift up experiments in continuous culture to gain a better insight into cellular metabolism and its redistribution.

TL;DR: This very simple encapsulation of penicillin G acylase into a very rigid polymeric matrix based on polyvinyl alcohol (LentiKats) provides an efficient technique for solving the mechanical stability problem associated with CLEA, but also greatly improves the behavior of CLEA in organic media.