H
Herbert L. Heyneker
Researcher at Genentech
Publications - 88
Citations - 20004
Herbert L. Heyneker is an academic researcher from Genentech. The author has contributed to research in topics: Escherichia coli & Gene. The author has an hindex of 36, co-authored 88 publications receiving 19856 citations. Previous affiliations of Herbert L. Heyneker include University of California, San Francisco & Genencor.
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Patent
Recombinant immunoglobin preparations
TL;DR: In this paper, a mixture of altered and native immunoglobulins, including constant-variable region chimeras, are prepared in recombinant cell culture, which contain variable regions which are immunologically capable of binding predetermined antigens.
Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli
Diane Pennica,William E. Holmes,William J. Kohr,Richard N. Harkins,Gordon A. Vehar,Carole A. Ward,William F. Bennett,Elizabeth Mcleod Yelverton,Peter H. Seeburg,Herbert L. Heyneker,David V. Goeddel,Desire Collen +11 more
TL;DR: Bacterial clones containing human tissue-type plasminogen activator cDNA sequences were identified in a cDNA library prepared using gel-fractionated mRNA from human melanoma cells and a polypeptide was produced having the fibrinolytic properties characteristic of authentic human t-PA.
Journal ArticleDOI
Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin
Keiichi Itakura,Tadaaki Hirose,Roberto Crea,Arthur D. Riggs,Herbert L. Heyneker,Francisco Bolívar,Herbert W. Boyer +6 more
TL;DR: This work has reported the first synthesis of a functional polypeptide product from a gene of chemically synthesized origin, including the sequence of amino acids corresponding to somatostatin, in vitro.
Patent
Recombinant immunoglobulin preparations, methods for their preparation, DNA sequences, expression vectors and recombinant host cells therefor
TL;DR: Recombinant DNA techniques are used to produce both immunoglobulins which are analogous to those normally found in vertebrate systems and to take advantage of these gene modification techniques to construct chimeric or other modified forms.