Journal ArticleDOI
Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
TLDR
In vitro recombinant DNA techniques were used to construct two new cloning vehicles, pBR324 and pBR235, which permit the molecular cloning and easy selection of EcoRI, BamHI, HindIII, PstI, HincII, SalI, (XamI), Smal, ( XmaI), BglII and DpnII restriction generated DNA molecules.About:
This article is published in Gene.The article was published on 1978-10-01. It has received 708 citations till now. The article focuses on the topics: Plasmid & PBR322.read more
Citations
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Journal ArticleDOI
A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria
TL;DR: In this paper, a new vector strategy for the insertion of foreign genes into the genomes of gram negative bacteria not closely related to Escherichia coli was developed, which can utilize any gram negative bacterium as a recipient for conjugative DNA transfer.
Journal ArticleDOI
The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector
TL;DR: It was found that the gene 5 promoter is active in a tissue-specific fashion whereas this is not the case for the NOS promoter, providing the first documented instance of a gene derived from a procaryotic host the expression of which is apparently regulated by plant growth factors.
Journal ArticleDOI
Isolation of the Candida albicans gene for orotidine-5'-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations.
TL;DR: A gene bank of Sau3A partially digested Candida albicans DNA in vector YEp13 was used to complement a ura3 mutation, OMPdecase, which was sufficient to allow complementation in S. cerevisiae with integrating as well as high copy number vectors.
Journal ArticleDOI
Expression of genes transferred into monocot and dicot plant cells by electroporation
TL;DR: Gene-transfer efficiency increased with the DNA concentration and was affected by the amplitude and duration of the electric pulse as well as by the composition of the electroporation medium, demonstrating that the method is applicable to both monocot and dicot protoplasts.
Journal ArticleDOI
Microbial phenazine production enhances electron transfer in biofuel cells
TL;DR: The finding that one bacterium can produce electron shuttles, which can be used also by other bacteria, to enhance electron-transfer rate and growth, has not been shown before and has considerable implications with respect to the power output attainable in MFCs.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences
Axel Ullrich,John Shine,Chirgwin John Mitchell,Raymond Pictet,Edmund Tischer,William J. Rutter,Howard M. Goodman +6 more
TL;DR: Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA that contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin II prepeptide, and the untranslated 3' terminal region of the mRNA.
Journal ArticleDOI
Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
TL;DR: The colicinogenic factor E(1) (Col E( 1)) in Escherichia coli continues to replicate by a semiconservative mechanism in the presence of chloramphenicol for 10 to 15 hr, long after chromosomal deoxyribonucleic acid (DNA) synthesis has terminated.
Journal ArticleDOI
Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
TL;DR: In vitro recombination via restriction endonucleases and the in vivo genetic translocation of the Ap resistance (Apr) gene resulted in the construction of a new cloning vehicle, the plasmid pBR313, which has a molecular weight of 5.8 Mdalton and has been characterized using thirteen restriction enzymes, six of which cleave the plasid at unique restriction sites.