抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

Coherence transfer by isotropic mixing: Application to proton correlation spectroscopy

A two-dimensional nuclear overhauser experiment with pure absorption phase in four quadrants☆

https://zenodo.org/record/45375/files/article.pdf

Natural abundance nitrogen-15 NMR by enhanced heteronuclear spectroscopy

The widespread use of controlled molecular-level motion in key natural processes suggests that great rewards could come from bridging the gap between the present generation of synthetic molecular systems, which by and large rely upon electronic and chemical effects to carry out their functions, and the machines of the macroscopic world, which utilize the synchronized movements of smaller parts to perform specific tasks. This is a scientific area of great contemporary interest and extraordinary recent growth, yet the notion of molecular-level machines dates back to a time when the ideas surrounding the statistical nature of matter and the laws of thermodynamics were first being formulated. Here we outline the exciting successes in taming molecular-level movement thus far, the underlying principles that all experimental designs must follow, and the early progress made towards utilizing synthetic molecular structures to perform tasks using mechanical motion. We also highlight some of the issues and challenges that still need to be overcome.

Synthetic molecular motors and mechanical machines.

Binding of a Porphyrin Conjugate of Hoechst 33258 to DNA. Part 2. NMR Spectroscopic Studies Detect Multiple Binding Modes to a 12‐mer Nonself‐Complementary Duplex DNA.

Suppression of 13C satellites in 1H DOSY spectra.

The 1H and 13C NMR spectra of erythromycin A in [2H6]-DMSO and buffered D2O have been assigned using a range of one and two dimensional NMR techniques. Assignments have been made for both the major (keto) and minor forms of the drug, and the minor form has been shown to be the 12,9-cyclic hemiacetal.

The Structure of Erythromycin A in (2H6) DMSO and Buffered D2O: Full Assignments of the 1H and 13C NMR Spectra

Analysis of foods, which are typically highly complex mixtures, by 1H NMR can be difficult because the prevalence of signal overlap complicates characterization and quantification. The various components of a food sample may have a wide range of concentrations, leading to a high dynamic range NMR spectrum and complicating the analysis of less concentrated species. One source of this complication is the presence of 13C satellites, peaks that appear either side of a parent peak with ∼0.56% of its intensity. Satellites of concentrated species can easily be comparable in intensity to the signals of minor components, and can partly or wholly obscure them. This is commonly seen in olive oil samples, leading to inaccurate calculation of the fatty acid ester composition of the oil, used for determining the quality of edible oils and for detecting adulteration. Here, we show that the recently introduced Destruction of Interfering Satellites by Perfect Echo Low-pass filtration (DISPEL) experiment is able to suppress 13C satellites and can substantially improve the accuracy of integration of minor signals. The DISPEL experiment does not require any complicated optimization, working “out of the box” with standard parameters, and incurs no significant loss of sensitivity. It has the potential to become the default experiment, replacing conventional 1D 1H NMR, for quantitative analysis of olive oil.

https://pubs.acs.org/doi/pdf/10.1021/acsfoodscitech.2c00057

Improved Quantification by Nuclear Magnetic Resonance Spectroscopy of the Fatty Acid Ester Composition of Extra Virgin Olive Oils

Synthesis of (1,2-Benzenediolato(2-)-O,O′)oxobis(1-piperidinolato(1-)-O,N)molybdenum(VI), [MoO(C5H10NO)2(C6H4O2)], and Structure Determination by Correlated1H -13C Two-Dimensional NMR Spectroscopy and X-Ray Crystallography.

Gareth A. Morris

Papers

Binding of a Porphyrin Conjugate of Hoechst 33258 to DNA. Part 2. NMR Spectroscopic Studies Detect Multiple Binding Modes to a 12‐mer Nonself‐Complementary Duplex DNA.

Suppression of 13C satellites in 1H DOSY spectra.

The Structure of Erythromycin A in (2H6) DMSO and Buffered D2O: Full Assignments of the 1H and 13C NMR Spectra

Improved Quantification by Nuclear Magnetic Resonance Spectroscopy of the Fatty Acid Ester Composition of Extra Virgin Olive Oils

Synthesis of (1,2-Benzenediolato(2-)-O,O′)oxobis(1-piperidinolato(1-)-O,N)molybdenum(VI), [MoO(C5H10NO)2(C6H4O2)], and Structure Determination by Correlated1H -13C Two-Dimensional NMR Spectroscopy and X-Ray Crystallography.